Ganoderma Lucidum Polysaccharide, an Extract from Ganoderma Lucidum, Exerts Suppressive Effect on Cervical Cancer Cell Malignancy through Mitigating Epithelial-Mesenchymal and JAK/STAT5 Signaling Pathway
Ganoderma Lucidum Polysaccharide, an Extract from Ganoderma Lucidum, Exerts Suppressive Effect on Cervical Cancer Cell Malignancy through Mitigating Epithelial-Mesenchymal and JAK/STAT5 Signaling Pathway
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DOI：
10.1159/000505461
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发表时间：
2020-07-01
期刊：
影响因子：
3.1
通讯作者：
Zhou, Guodong
中科院分区：
文献类型：
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作者：
Jin, Hui;Song, Chunye;Zhou, Guodong
目的：探讨灵芝多糖（GLP）对宫颈癌细胞的抗肿瘤作用。方法：采用不同浓度的GLP处理宫颈癌细胞，观察GLP对宫颈癌细胞生长的影响。细胞计数kit-8法的数据证明GLP的最佳工作浓度和时间为200 μ g/mL，处理48 h。transwell实验表明GLP能抑制宫颈癌细胞的侵袭和迁移能力。流式细胞仪检测结果显示GLP可促进宫颈癌细胞凋亡，并对宫颈癌细胞周期有一定的限制作用。Western blot检测发现，GLP处理后，促凋亡蛋白Bax、Cleaved Caspases 3和9的表达增加，抗凋亡蛋白Bcl-2的表达减少。此外，我们发现E-cadherin的表达增加，而N-cadherin，Vimentin和Slug的表达减少。结论：GLP能抑制宫颈癌细胞的活性和侵袭性，阻滞细胞周期，促进细胞凋亡，其机制可能与抑制上皮-间质和JAK/STAT 5通路有关。
Background/Aims:We aimed to explore whether ganoderma lucidum polysaccharide (GLP) exhibits antitumor effect on cervical cancer cells.Methods and Results:Different concentration of GLP was used to treat cervical cell. The data from cell counting kit-8 assay proved that the optimal working concentration and time of GLP were 200 mu g/mL and treated for 48 h. The transwell assay demonstrated that GLP could attenuate the invasion and migration abilities of cervical cancer cells. Moreover, flow cytometry illustrated that GLP could promote the apoptosis of cervical cancer cells and limit the cycle of cervical cancer cells. Western blot assay discovered that the expression of proapoptosis proteins including Bax, Cleaved Caspases 3 and 9 increased and the antiapoptosis protein Bcl-2 decreased after treated with GLP. Moreover, we found that the expression of E-cadherin was increased, and N-cadherin, Vimentin, and Slug were decreased. Meanwhile, the expression of phosphorylated-JAK and phosphorylated-STAT5 was also decreased in cervical cancer cells treated by GLP, suggesting the inhibitory effect on JAK/STAT5 pathways.Conclusions:All of these data illustrated that GLP could alleviate the activity and aggressiveness, block the cell cycle, and promote the apoptosis of cervical cancer cells, which were possible via inhibiting epithelial-mesenchymal and JAK/STAT5 pathways.