Extracellular matrix inhibits structural and functional plasticity of dendritic spines in the adult visual cortex
Extracellular matrix inhibits structural and functional plasticity of dendritic spines in the adult visual cortex
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DOI：
10.1038/ncomms2491
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发表时间：
2013-02-01
影响因子：
16.6
通讯作者：
Ratto, G. M.
中科院分区：
文献类型：
--
作者：
de Vivo, L.;Landi, S.;Ratto, G. M.
脑细胞沉浸在形成细胞外基质的复杂结构中。在出生后的发育过程中，随着大脑回路达到其成人形式，基质的组成逐渐成熟。充分发育的细胞外环境稳定神经元连接并降低皮质可塑性，如通过降解基质的治疗能够恢复成年大脑中的突触可塑性的证明所强调的。基质抑制皮质可塑性的机制尚未完全阐明。在这里，我们表明，一个突出的组成部分的矩阵，硫酸软骨素蛋白聚糖（CSPGs），抑制形态学变化的树突棘在成年小鼠的视觉皮层。通过在体和离体双光子成像和电生理学研究，我们发现CSPGs酶消化后，皮质棘变得更活跃，表现出更大程度的结构和功能可塑性。
Brain cells are immersed in a complex structure forming the extracellular matrix. The composition of the matrix gradually matures during postnatal development, as the brain circuitry reaches its adult form. The fully developed extracellular environment stabilizes neuronal connectivity and decreases cortical plasticity as highlighted by the demonstration that treatments degrading the matrix are able to restore synaptic plasticity in the adult brain. The mechanisms through which the matrix inhibits cortical plasticity are not fully clarified. Here we show that a prominent component of the matrix, chondroitin sulfate proteoglycans (CSPGs), restrains morphological changes of dendritic spines in the visual cortex of adult mice. By means of in vivo and in vitro two-photon imaging and electrophysiology, we find that after enzymatic digestion of CSPGs, cortical spines become more motile and express a larger degree of structural and functional plasticity.