EFFECTS OF CIGARETTE-SMOKE EXTRACT ON BOVINE BRONCHIAL EPITHELIAL-CELL ATTACHMENT AND MIGRATION
EFFECTS OF CIGARETTE-SMOKE EXTRACT ON BOVINE BRONCHIAL EPITHELIAL-CELL ATTACHMENT AND MIGRATION
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DOI：
10.1152/ajplung.1995.268.5.l723
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发表时间：
1995-05-01
影响因子：
4.9
通讯作者：
SPURZEM, JR
中科院分区：
文献类型：
--
作者：
CANTRAL, DE;SISSON, JH;SPURZEM, JR
受损上皮的修复涉及上皮细胞和下面的细胞外基质之间复杂的相互作用。我们研究了亚致死浓度的香烟烟雾提取物（CSE）和香烟烟雾中的两种挥发性成分乙醛和丙烯醛对体外牛支气管上皮细胞（BBEC）附着和迁移的影响。短期暴露于 CSE 后（2 小时和 6 小时），BBEC 对纤连蛋白包被培养皿的附着减少，并且向纤连蛋白的迁移没有变化。经过较长时间的暴露（24 小时）后，附着力增加，迁移没有变化。暴露于含有氨基酸序列精氨酸-甘氨酸-天冬氨酸（RGD）的肽中，对照细胞和暴露于烟雾的细胞同样能减少附着。暴露于 CSE 后 72 小时内，BBEC 片迁移减少。丙烯醛减少 BBEC 向纤连蛋白的迁移，但对附着没有影响。乙醛对附着或迁移没有影响。我们得出的结论是，暴露于 CSE 对支气管上皮细胞迁移和附着具有重要影响，并且这些影响随着时间的推移而变化。
The repair of injured epithelium involves a complex interaction between epithelial cells and the underlying extracellular matrix. We studied the effects of sublethal concentrations of cigarette smoke extract (CSE) and two volatile components of cigarette smoke, acetaldehyde and acrolein, on bovine bronchial epithelial cell (BBEC) attachment and migration in vitro. After short-term exposure (2 and 6 h) to CSE, BBEC attachment to fibronectin-coated dishes was decreased, and migration to fibronectin was unchanged. After a longer period of exposure, 24 h, attachment was increased and migration was unchanged. Exposure to a peptide containing the amino acid sequence arginine-glycine-aspartic acid (RGD) reduced attachment equally well for control and smoke-exposed cells. BBEC sheet migration was reduced over 72 h after exposure to CSE. Acrolein reduced BBEC migration to fibronectin but had no effect on attachment. Acetaldehyde had no effect on either attachment or migration. We conclude that exposure to CSE has important effects on bronchial epithelial cell migration and attachment, and that these effects change over time.