Thy 1+ dendritic epidermal cells but not Langerhans cells express Ly 48.
Thy 1+ dendritic epidermal cells but not Langerhans cells express Ly 48.
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Thy 1 树突状表皮细胞表达 Ly 48，但朗格汉斯细胞不表达。
DOI：
10.1111/1523-1747.ep12469016
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Frelinger,JG
中科院分区：
文献类型：
--
作者：
Gaspari,AA;Ferbel,B;Frelinger,JG
Leukosialin(Ly 48)是一种唾液酸蛋白，由骨髓源性细胞在发育早期表达。为了确定Ly 48是由特定的表皮细胞亚群(如朗格汉斯细胞、角质形成细胞或树突状表皮T细胞)表达，我们利用流式细胞术检测了一组识别白蛋白抗原两个不同表位的四种单抗(S11、S15、S7和3E8)对ECs的染色。约2.0%的未分离内皮细胞表达Ly 48，与单抗ST11和S15的反应性证明。同样，未分离的EC裂解物的免疫印迹显示，一个125kD的表观分子量的抗原与S11单抗发生反应。不同EC种群的富集性或衰减性表明，DETCs是唯一表达Ly 48的EC种群。对长期培养的DETC株系的研究表明，Ly48表位的表达具有明显的异质性。Ly 48对DETCs和T细胞受体α/β的作用(S)尚未确定。
Leukosialin (Ly 48) is a sialoprotein expressed by bone marrow – derived cells early in their development. To determine whether Ly 48 is expressed by specific subsets of epidermal cells (ECs) such as Langerhans cells, keratinocytes, or dendritic epidermal T cells (DETCs), we utilized flow cytometry to detect staining of ECs by a panel of four monoclonal antibodies (S11, S15, S7, and 3E8) that recognize two different epitopes of the leukosialin antigen. Approximately 2.0% of unfractionated ECs expressed Ly 48, as demonstrated by reactivity with the monoclonal antibodies St11 and S15. Similarly, immunoblots of unfractionated EC lysates revealed an antigen of 125 kD apparent molecular weight that reacted with the S11 monoclonal antibody. Enrichment or depletion of various EC populations indicated that DETCs are the only EC population that express Ly 48. Studies of long-term cultured DETC lines indicated a marked heterogeneity of expression of Ly 48 epitopes. The function(s) of Ly 48 on DETCs as well as T-cell receptor α/β bearing mouse T cells remains to be determined.