Regulation of microtubule assembly by human EB1 family proteins
Regulation of microtubule assembly by human EB1 family proteins
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DOI：
10.1038/sj.onc.1204429
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发表时间：
2001-05-31
期刊：
影响因子：
8
通讯作者：
Su, LK
中科院分区：
文献类型：
--
作者：
Bu, W;Su, LK
EB1家族蛋白是一种高度保守的微管相关蛋白，在酵母中对微管动力学和染色体分离起着重要的调控作用。人EB1家族蛋白包括EB1、RP1和EBF3。虽然EB1和RP 1已被证明与微管相关，但内源性EBF3的亚细胞定位尚未被表征。人EB 1家族蛋白的功能也不清楚。因此，我们研究了EBF3的细胞定位和EB1家族蛋白对微管组织的调节。与EB1和RP 1一样，发现EBF3在整个细胞周期中与微管共定位，优先在其正端。此外，在间期细胞的中心体和有丝分裂细胞的纺锤体极处有非常强的EBF3信号。当EB1家族蛋白过表达时，它们与整个微管细胞骨架结合，此外，EB1和EBF3在过表达这些蛋白的一些细胞中诱导微管成束。这些微管束对诺考达唑的抗性更强，并且比常规微管乙酰化程度更高。我们的研究结果首次表明，人EB 1家族蛋白可以调节微管组装和稳定性。
The EB1 family proteins are highly conserved microtubule-associated proteins, The EB1 protein in yeast has been shown to play an important role in regulating microtubule dynamics and chromosome segregation. Human EB1 family proteins include EB1, RP1 and EBF3. Although EB1 and RP1 have been shown to associate with microtubules, the subcellular localization of endogenous EBF3 had not been characterized. The function of human EB1 family proteins was also not clear. We therefore investigated the cellular localization of EBF3 and the regulation of microtubule organization by EB1 family proteins. As do EB1 and RP1, EBF3 was found to colocalize with microtubules, preferentially at their plus ends, throughout the cell cycle. Moreover, there was a very strong EBF3 signal at the centrosome in interphase cells and at the spindle poles in mitotic cells. When EB1 family proteins were overexpressed, they associated with the entire microtubule cytoskeleton, In addition, EB1 and EBF3 induced microtubule bundling in some cells overexpressing these proteins. These microtubule bundles were more resistant to nocodazole and were more acetylated than regular microtubules. Our results demonstrate for the first time that human EB1 family proteins could regulate microtubule assembly and stability.