Auditory epithelial migration .3. An immunohistologic study using anti-BrdU antibody on tympanic membrane in mouse
Auditory epithelial migration .3. An immunohistologic study using anti-BrdU antibody on tympanic membrane in mouse
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DOI：
10.1177/000348949710600510
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发表时间：
1997-05-01
影响因子：
1.4
通讯作者：
Anniko, M
中科院分区：
文献类型：
--
作者：
Kakoi, H;Anniko, M
本文用抗溴脱氧尿苷（BrdU）抗体的免疫组织化学技术，研究了小鼠鼓膜上皮细胞的定位及其迁移方式。腹膜内滴注BrdU，并在注射后1小时至10天之间无痛处死动物。使用抗BrdU抗体的免疫染色技术应用于整个安装TM组织。注射后1小时，在锤骨（HM）区的柄部和TM紧张部的环形区发现BrdU标记的细胞。在上级象限上半部分的中间区观察到一些标记细胞，但在中间区的其余部分未发现标记细胞。标记细胞在松弛部也很明显，没有任何特定的分布模式。随着注射后时间的推移，首先出现在HM区的标记细胞已经从HM向环横向和纵向迁移，而环区的标记细胞数量明显减少。本研究的结果如下：1）张力部表皮细胞的增殖中心位于两个不同的区域，即HM区和环形区，2）HM区的新生细胞从HM区向环形区迁移，而环形区的新生细胞从环形区向外耳道迁移，（3）松弛部无特定的发生中心。在此基础上，我们讨论了表皮细胞增殖中心的位置与其迁移方式之间的关系。
A localization pattern of epidermal cells on the tympanic membrane (TM) and their migratory patterns were studied in mice, by means of immunohistologic technique using an anti-bromodeoxyuridine (BrdU) antibody. The BrdU was instilled intraperitoneally and the animals were painlessly sacrificed between 1 hour and 10 days after the injection. An immunostaining technique using anti-BrdU antibodies was applied on whole mount TM tissues. One hour after injection, BrdU-labeled cells were found in the handle of the malleus (HM) region and in the annular region of the pars tense of the TM. Some labeled cells were observed in the intermediate region of the upper half of the superior quadrant, but no labeled cells were found in the remaining part of the intermediate region. Labeled cells were also evident in the pars flaccida without any particular pattern of distribution. As time elapsed after the injection, the labeled cells first appearing in the HM region had migrated laterally and inferiorly from the HM toward the annulus, while those in the annular-region had considerably decreased in number. Results of the present study are the following: 1) the proliferation center of epidermal cells in the pars tensa is located in two different areas, ie, the HM region and annular region, 2) newly generated cells in the HM region migrate from the HM region toward the annular region, whereas those in the annular region migrate from the annular region to the external auditory canal, and 3) no specific generation centeris located in the pars flaccida. On the basis of these results, we discuss the relationship between the site of the proliferation center of epidermal cells and their migratory patterns.