PHOTIC AND CIRCADIAN REGULATION OF C-FOS-GENE EXPRESSION IN THE HAMSTER SUPRACHIASMATIC NUCLEUS
PHOTIC AND CIRCADIAN REGULATION OF C-FOS-GENE EXPRESSION IN THE HAMSTER SUPRACHIASMATIC NUCLEUS
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DOI：
10.1016/0896-6273(90)90303-w
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发表时间：
1990-08-01
期刊：
影响因子：
16.2
通讯作者：
TAKAHASHI, JS
中科院分区：
文献类型：
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作者：
KORNHAUSER, JM;NELSON, DE;TAKAHASHI, JS
光信息携带位于哺乳动物下丘脑视交叉上核（SCN）的昼夜节律起搏器，以适应环境光/暗循环。为了确定光是否调节SCN中c-fos基因的表达，我们测量了金仓鼠SCN中c-fos mRNA的水平。我们报告说，在主观的夜晚，光线导致SCN中c-fos mRNA水平的迅速增加。持续5分钟的光脉冲足以诱导c-fos mRNA，并且在Iii开始后30分钟出现最高mRNA水平。诱导c-fos mRNA增加所需的最低光照水平与产生仓鼠昼夜节律活动相移的最低光照水平难以区分。此外，光对SCN中c-fos mRNA的诱导本身也受昼夜节律调节。c-fos mRNA的光诱导仅发生在主观夜晚，在昼夜节律时间，光相活动发生转移。综上所述，这些数据表明c-fos可能是哺乳动物昼夜节律的光通路的分子组成部分。
Photic information entrains a circadian pacemaker Iocated in the suprachiasmatic nucleus (SCN) of the mammalian hypothalamus to environmental light/dark cycles To determine whether light regulates c-fos gene expression in the SCN, we have measured c-fos mRNA levels in the SCN of the golden hamster. We report that, during the subjective night, light causes a rapid increase in levels of c-fos mRNA in the SCN. light pulses of 5 min duration are sufficient to induce c-fos mRNA, and the highest mRNA levels occur 30 min following the onset of Iii. The minimum level of illumination required to inducean increase in c-fos mRNA is indistinguishable from the minimum irradiance that produces a phase shift in the hamster’s circadian rhythm of activity. In addition, the induction of c-fos mRNA in the SCN by light is itself under circadian regulation. Light induction of c-fos mRNA occurs only during the subjective night, at circadian times when photic phase shifting of activity occurs. Taken together, these data suggest that c-fos may be a molecular component of the photic pathway for entrainment of mammalian circadian rhythms.