The bracteatus pineapple genome and domestication of clonally propagated crops
The bracteatus pineapple genome and domestication of clonally propagated crops
复制标题
菠萝基因组和克隆繁殖作物的驯化
DOI：
10.1038/s41588-019-0506-8
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发表时间：
2019-10-01
期刊：
影响因子：
30.8
通讯作者：
Ming, Ray
中科院分区：
文献类型：
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作者：
Chen, Li-Yu;VanBuren, Robert;Ming, Ray
对南美菠萝等无性系繁殖作物的驯化被认为是一步到位的操作。我们测定了Ananas comosus var.Brentteatus CB5，将513Mb组装成25条染色体，含有29,412个基因。通过对CB5、F153和MD2的基因组比较，揭示了纤维形成、色泽形成、糖分积累和果实成熟的基因组基础。我们还对89个Ananas基因组进行了重新测序。品种‘光滑卡宴’和‘皇后’表现出古代和现代的混合，而‘新加坡西班牙’则支持一步驯化。我们确定了25次选择性扫描，其中包括一次含有一对复制菠萝酶抑制剂的强扫描。自交不亲和的4个候选基因在F153中连锁，但在自交不亲和的CB5中不起作用。我们的发现支持在无性系繁殖作物的驯化中，有性重组和一步操作并存。这项工作指导探索其他无性系繁殖作物的有性和无性驯化轨迹。
Domestication of clonally propagated crops such as pineapple from South America was hypothesized to be a 'one-step operation'. We sequenced the genome of Ananas comosus var. bracteatus CB5 and assembled 513 Mb into 25 chromosomes with 29,412 genes. Comparison of the genomes of CB5, F153 and MD2 elucidated the genomic basis of fiber production, color formation, sugar accumulation and fruit maturation. We also resequenced 89 Ananas genomes. Cultivars 'Smooth Cayenne' and 'Queen' exhibited ancient and recent admixture, while 'Singapore Spanish' supported a one-step operation of domestication. We identified 25 selective sweeps, including a strong sweep containing a pair of tandemly duplicated bromelain inhibitors. Four candidate genes for self-incompatibility were linked in F153, but were not functional in self-compatible CB5. Our findings support the coexistence of sexual recombination and a one-step operation in the domestication of clonally propagated crops. This work guides the exploration of sexual and asexual domestication trajectories in other clonally propagated crops.