Convergence of Dip-Pen Nanolithography and acoustic biosensors towards a rapid-analysis multi-sample microsystem
Convergence of Dip-Pen Nanolithography and acoustic biosensors towards a rapid-analysis multi-sample microsystem
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DOI：
10.1039/c2an35156k
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Gizeli, Electra
中科院分区：
文献类型：
--
作者：
Mitsakakis, Konstantinos;Sekula-Neuner, Sylwia;Gizeli, Electra
本工作首次展示了使用Dip-Pen Nanolithography（DPN）的具有几种不同生物分子的即用型生物传感器的图案化，用于开发更快速和有效的多样品检测程序。所使用的生物传感器平台是基于表面声波（SAW）设备集成的平行通道微流体模块，称为“微流体上SAW”（“μ F-on-SAW”），用于可再现的多样品分析。具有不同官能化头部基团的脂质在不同的微流体形成的矩形域处图案化，所述矩形域具有全部位于相同传感器表面上的尖锐边缘;使用荧光显微镜验证图案质量。随后通过声学实验检查头基团的功能性、图案化方法的效率以及DPN用于声学装置的表面改性的适用性。muF-on-SAW配置用于检测预图案化的功能化脂质与其相应的生物分子之间的特异性结合。实现改进的灵敏度（5倍相比，以前的声学配置）和减少至少2小时的制备时间清楚地表明DPN作为一个直接的图案化方法，如μ F-SAW对集成，快速分析，多样品生物传感微系统的发展准备使用的声学传感器设备的适用性。
The present work demonstrates for the first time patterning of a ready-to-use biosensor with several different biomolecules using Dip-Pen Nanolithography (DPN) for the development of a procedure towards more rapid and efficient multi-sample detection. The biosensor platform used is based on a Surface Acoustic Wave (SAW) device integrated with a parallel-channel microfluidic module, termed as "microfluidics-on-SAW" ("mu F-on-SAW"), for reproducible multi-sample analysis. Lipids with different functionalized head groups were patterned at distinct, microfluidic-formed rectangular domains with sharp edges all located on the same sensor surface; pattern quality was verified using a fluorescent microscope. The functionality of the head groups, the efficiency of the patterning method, and the suitability of DPN for the surface modification of the acoustic device were subsequently examined through acoustic experiments. The mu F-on-SAW configuration was used to detect specific binding between the pre-patterned functionalized lipids with their corresponding biomolecules. The achievement of an improved sensitivity (5-fold compared to previous acoustic configurations) and reduced preparation time by at least 2 h clearly indicates the suitability of DPN as a direct patterning method for ready-to-use acoustic sensor devices like the mu F-on-SAW towards integrated, rapid-analysis, multi-sample biosensing microsystem development.