Correlation of diffusion MRI and heat shock protein in a rat embolic stroke model
Correlation of diffusion MRI and heat shock protein in a rat embolic stroke model
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DOI：
10.1016/s0022-510x(97)05368-9
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发表时间：
1997-05-29
影响因子：
4.4
通讯作者：
Welsh, FA
中科院分区：
文献类型：
--
作者：
Detre, JA;Zager, EL;Welsh, FA
70kda热休克蛋白（HSP-70）在局灶性缺血中的表达发生在亚致死性缺血损伤区域，因此可能被认为是缺血半暗带的生物学标志。在大鼠栓塞性中风模型中，我们使用富含纤维蛋白的栓子，将HSP-70 mRNA的表达与扩散磁共振成像（MRI）相关联，以确定HSP-70 mRNA的表达是否与脑组织水表观扩散系数（ADC）的改变有关，ADC是一种易于在体内测量的细胞毒性损伤的早期标志。在右颈动脉区域栓塞性梗死后120分钟内进行连续ADC测量。HSP-70 mRNA在密集缺血区边界表达，通过弥散成像判断。HSP-70 mrna阳性区ADC值介于缺血核心区和对照区之间。此外，HSP-70 mrna阳性组织的体积与120 min时显示中等ADC值的组织体积呈正相关。这些结果表明中等ADC值出现在半影区。缺血性细胞损伤的异质性被认为是这些区域观察到的中间ADC值的基础。(C) 1997爱思唯尔科学有限公司
The expression of 70 kDa heat shock protein (HSP-70) in focal ischemia occurs in regions that sustain sub-lethal ischemic injury, and may therefore be considered as a biological marker of the ischemic penumbra. In a rat embolic stroke model, using fibrin-rich emboli, we correlated the expression of HSP-70 mRNA with diffusion magnetic resonance imaging (MRI) to determine if HSP-70 mRNA expression was associated with alterations in the apparent diffusion coefficient (ADC) of brain tissue water, a putative early marker of cytotoxic injury that is readily measured in vivo. Serial ADC measurements were made for 120 min following embolic infarction in the right carotid artery territory. HSP-70 mRNA expression was observed at the boundaries of the densely ischemic zone, as judged by diffusion imaging. ADC values observed in HSP-70 mRNA-positive regions were intermediate between those observed in the ischemic core or in control regions. In addition, the volume of HSP-70 mRNA-positive tissue correlated positively with the volume of tissue showing intermediate ADC values at 120 min. These findings suggest that intermediate ADC values occur in penumbral regions. Heterogeneity of ischemic cellular injury is suggested as the basis for the intermediate ADC values observed in these regions. (C) 1997 Elsevier Science B.V.