First Evidence for Family-Specific QTL for Temperature-Dependent Sex Reversal in Nile Tilapia (Oreochromis niloticus)
First Evidence for Family-Specific QTL for Temperature-Dependent Sex Reversal in Nile Tilapia (Oreochromis niloticus)
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DOI：
10.1159/000339705
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发表时间：
2012-01-01
影响因子：
2.3
通讯作者：
Wessels, S.
中科院分区：
文献类型：
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作者：
Luehmann, L. M.;Knorr, C.;Wessels, S.
本研究首次筛选了与尼罗罗非鱼温度依赖性性别相关的微卫星标记。以往的研究表明，标记连锁群（LG）1，3，和23被连锁的表型性别的奥利亚罗非鱼。在正常的饲养温度下。此外，性别决定和分化的候选基因已被定位到这些连锁群。在这里，6个家庭的温度处理的遗传全女性（XX）的F-1-人口的基因型为21个微卫星的3个LG。没有群体范围的QTL（数量性状位点）或标记性状协会可以检测到。而在UNH 995和UNH 104两侧的LG 1、GM 213位置的LG 3和GM 283旁边的LG 23上发现了家族特异性QTL。此外，在LG 1上检测到UNH 995和UNH 104，在LG 3上检测到GM 213，以及在LG 23上检测到UNH 898和GM 283的家族特异性单标记关联。然而，标记性状协会不能解释的温度依赖性性别的所有鱼类在各自的家庭。尼罗罗非鱼温度依赖性性别的分子线索可能部分符合等位基因变异的主要和次要遗传性别决定因素。此外，额外的QTL有助于对温度的可变负债可能会持续对其他LG。版权所有（C）2012 S. Karger AG，巴塞尔
This study for the first time screens microsatellite markers for associations with the temperature-dependent sex of Oreochromis niloticus. Previous studies revealed markers on linkage groups (LG) 1, 3, and 23 to be linked to the phenotypic sex of Oreochromis spp. at normal rearing temperatures. Moreover, candidate genes for sex determination and differentiation have been mapped to these linkage groups. Here, 6 families of a temperature-treated genetically all-female (XX) F-1-population were genotyped for 21 microsatellites on the 3 LGs. No population-wide QTL (quantitative trait loci) or marker trait associations could be detected. However, family-specific QTL were found on LG 1 flanked by UNH995 and UNH104, on LG 3 at the position of GM213, and on LG 23 next to GM283. Moreover, family-specific single marker associations for UNH995 and UNH104 on LG 1, GM213 on LG 3, as well as for UNH898 and GM283 on LG 23 were detected. Yet, marker trait associations could not explain the temperature-dependent sex of all fish in the respective families. The molecular cue for the temperature-dependent sex in Nile tilapia might partially coincide with allelic variants at major and minor genetic sex determining factors. Moreover, additional QTL contributing to variable liabilities towards temperature might persist on other LGs. Copyright (C) 2012 S. Karger AG, Basel