Specific gangliosides increase rapidly in rat liver following partial hepatectomy.
Specific gangliosides increase rapidly in rat liver following partial hepatectomy.
复制标题
部分肝切除术后，大鼠肝脏中的特定神经节苷脂迅速增加。
DOI：
10.1016/0006-291x(91)91465-o
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
Ullman,MD
中科院分区：
文献类型：
--
作者：
Fishman,JB;Cahill,M;Morin,P;McCrory,M;Bucher,NL;Ullman,MD
大鼠肝神经节苷脂（唾液酸含鞘糖脂）进行了分析，无论是部分肝切除术或假手术后的HPTLC和HPLC。通过HPTLC对全肝神经节苷脂的分析表明，在部分（68%）肝切除术后6小时内，与假手术和对照动物相比，GM1显著增加。到48 h时，GM 1进一步升高，多唾液酸神经节苷脂GD 1a、GD 1b和GT 1b也显著升高，而GM 3变化不明显。通过HPLC评估，与假肝切除对照相比，再生肝脏中与质膜相关的神经节苷脂增加至3.5倍。虽然膜神经节苷脂的升高与肝细胞增殖有关，但它们并不密切遵循生长曲线。神经节苷脂生物合成的变化的时间过程表明，再生肝脏中GM3合酶和GD3合酶的差异上调。
Rat liver gangliosides (sialic acid containing glycosphingolipids) were analyzed by HPTLC and HPLC following either partial hepatectomy or sham operation. Analysis of whole liver gangliosides by HPTLC demonstrated that within 6 h after partial (68%) hepatectomy, there was a significant increase in GM1compared to both sham and control animals. By 48 h, GM1was further increased and the polysialylgangliosides GD1a, GD1band GT1bhad also risen significantly, whereas changes in GM3were negligible. Gangliosides associated with the plasma membrane were increased up to 3.5-fold in regenerating liver compared to sham-hepatectomized controls as assessed by HPLC. Although elevations in membrane gangliosides were associated with hepatocyte proliferation, they did not closely follow the growth curve. The time course of changes in ganglioside biosynthesis suggests differential upregulation of GM3synthase and GD3synthase in regenerating livers.