Increased levels of macrophage colony-stimulating factor in the placenta and blood in preeclampsia
Increased levels of macrophage colony-stimulating factor in the placenta and blood in preeclampsia
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DOI：
10.1034/j.1600-0897.2002.1o035.x
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发表时间：
2002-01-01
影响因子：
3.6
通讯作者：
Inaba, N
中科院分区：
文献类型：
--
作者：
Hayashi, M;Hoshimoto, K;Inaba, N
问题：巨噬细胞集落刺激因子（M-CSF）被认为是胎盘生长和维持的必需细胞因子。我们评估是否M-CSF水平在胎盘和血液中的先兆子痫不同，从那些在正常pregnancy. Method的研究：受试者是37名孕妇携带单胎，其中19名妇女正常妊娠和18名妇女与先兆子痫。她们的平均孕龄为38周。分娩前采血，离心分离血清。分娩后立即切除胎盘的一个组织段。将冷冻的胎盘组织置于含有磷酸盐缓冲盐水的塑料管中。将组织完全均质化，然后离心。分离的上清液用于后续测定。结果：子痫前期患者胎盘M-CSF/TP及血M-CSF水平均显著高于正常孕妇（P < 0.05-0.01）。胎盘中M-CSF的增加支持免疫异常与先兆子痫病因有关的假设。
PROBLEM: Macrophage colony-stimulating factor (M-CSF) is considered an essential cytokine for placental growth and maintenance. We evaluated whether M-CSF levels in the placenta and blood in preeclampsia differed from those in normal pregnancies.METHOD OF STUDY: The subjects were 37 pregnant women carrying single fetuses, of whom 19 were women with normal pregnancies and 18 were women with precclampsia. Their average gestational age at entry was 38 weeks of gestation. Blood was collected before the onset of labor, and separated serum was obtained after centrifugation. A tissue segment of the placenta was cut immediately after delivery. The frozen placental tissue was placed in a plastic tube containing phosphate-buffered saline. The tissue was fully homogenized and then centrifuged. Separated supernatant was used for subsequent determination. M-CSF levels in separated serum were measured, and M-CSF and total protein (TP) levels in separated supernatant were also measured.RESULTS: Both M-CSF/TP levels in the placenta and M-CSF levels in blood were significantly higher (P < 0.05-0.01) in preeclampsia than in normal pregnancies.CONCLUSIONS: This is the first report concerning high placenta levels of M-CSF/TP in preeclampsia. Increased M-CSF in the placenta supports the hypothesis that immunological abnormalities contribute to the etiology of preeclampsia.