Capturing and Clinical Applications of Circulating Tumor Cells with Wave Microfluidic Chip
Capturing and Clinical Applications of Circulating Tumor Cells with Wave Microfluidic Chip
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DOI：
10.1007/s12010-019-03199-4
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发表时间：
2019-11-28
影响因子：
3
通讯作者：
Chen, Hongmei
中科院分区：
文献类型：
--
作者：
Chen, Hongmei
微流控芯片循环肿瘤细胞计数作为一种“液体活检”，对肿瘤预后判断具有重要意义。CTC携带与原始肿瘤相同的重要信息。集成微流控芯片结合亲和性和物理性为基础，如波芯片提供了一种新的方法来分离CTC。本研究进一步研究了波形芯片捕获CTC的临床应用。当细胞悬浮液穿过微柱阵列时，CTC从微柱组织的狭窄间隙中挤出。这种移动使得CTC获得相对于流体方向的倾斜速度。这种倾斜的速度将引导CTC被下一个阵列的较小相邻间隙捕获。同时，由于改性微柱的屏障作用，相互作用或摩擦时间更长。这些微柱将有效地实现抗原和抗体的结合。因此，抗体包被的和基于物理的分离可以在分离CTC中组合。捕获百分比集中在前几个阵列的理论和实验。对于人工患者血液，可以获得有效的捕获。临床上，CTC对于三个转移性人乳腺癌患者样品测试为阳性。该芯片有望成为临床肿瘤细胞计数的有效工具，进行抗癌药物的检测。
As a "liquid biopsy," circulating tumor cell (CTC) enumeration with microfluidic chips has great significance in cancer prognosis. CTCs carry significant information as the original tumor. Integrated microfluidic chips are combining with affinity- and physical-based such as wave chip offers a new way to segregate CTCs. In this work, we further study capturing clinical applications of CTCs with wave chip. When cell suspension moves across the microposts array, CTCs squeeze out from narrow gaps organized by microposts. This movement renders CTCs to obtain a tilted velocity to fluid direction. This tilted velocity would direct CTCs to be captured by the smaller neighboring gaps next array. Simultaneously, interaction or friction time is longer due to barrier of modified microposts. These microposts would be effective for realizing binding of antigen and antibody. Therefore, both antibody-coated and physical-based isolations could be combined in isolating CTCs. Capture percentage concentrated on the first several arrays is shown theoretically and experimentally. Efficient capture could be obtained for artificial patient blood. Clinically, CTCs were tested positive for three metastatic human breast cancer patient samples. This wave chip is prospectively to be a valid tool for clinical enumeration of CTCs, carrying out anti-cancer drug assay.