Acellular Dermal Matrix from Different Ages for Tissue Engineering Scaffold: Aged Prior to Young
Acellular Dermal Matrix from Different Ages for Tissue Engineering Scaffold: Aged Prior to Young
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用于组织工程支架的不同年龄的脱细胞真皮基质：衰老先于年轻
DOI：
10.1166/jbt.2016.1499
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发表时间：
2016-09-01
影响因子：
0.1
通讯作者：
Li，Qiang
中科院分区：
文献类型：
--
作者：
Zhang，Aijun;Chen，Feifei;Li，Qiang
脱细胞真皮基质（ADM）支架已被应用于一系列组织工程产品中。然而，由于不希望的再细胞化和血管化，ADM的使用经常与严重的并发症有关。本研究制备了不同月龄的ADM，并对其体外生物学行为和体内再细胞化及血管化进行了研究。我们比较了年轻皮肤和老年皮肤制成的真皮基质的孔隙率，发现老年真皮基质比年轻皮肤更疏松、多孔，孔径也更合适。体外ADM与成纤维细胞共培养实验显示，细胞从真皮表面向ADM内部延伸，老化ADM较年轻ADM更能支持细胞迁移。皮下移植中，老年阿霉素对成纤维细胞的穿透深度优于年轻阿霉素，炎症反应也比年轻阿霉素轻。Vimentin和CD31免疫荧光染色显示，老年ADM可被Vimentin和CD31阳性细胞迅速再细胞化，细胞穿透和毛细血管再生程度老年组高于青年组。总体而言，本研究提示老年ADM多孔性更好，再细胞化、血管化和免疫原性上级年轻ADM，可能是一种比年轻ADM更有利的组织工程支架。
Acellular dermal matrix (ADM) scaffolds have been used in a series of tissue engineered products. However, due to the undesirable recellularization and vascularization, the use of ADM has been frequently linked to significant complications. In the present study, we prepared ADM from different ages and examined the in vitro biological behavior and in vivo recellularization and vascularization. We compared the porosity of the ADM made from young and aged skin and found that aged ADM was more loose and porous than the young, the pore size was also more appropriate. The in vitro coculture test of ADM and fibroblast showed that cells extended into the inside of ADM from the dermis surface and aged ADM was more able to support cell migration than the young. In a subcutaneously transplantation, aged ADM exhibited advantage over young ADM in cell penetration depth of fibroblast, the inflammation reaction was also milder than the young. Moreover, immunofluorescence staining of vimentin and CD31 showed that aged ADM recellularized rapidly by vimentin and CD31 positive cells, the extent of cell penetration and capillary regeneration in old group was higher than the young. Overall, this study highlights that aged ADM is more porous, the recellularization, revascularization and immunogenicity properties are superior to the young, it may be a more favorable tissue engineering scaffold than the young.