Isolation and structural characterization of a polysaccharide LRP4-A from Lycium ruthenicum Murr.
Isolation and structural characterization of a polysaccharide LRP4-A from Lycium ruthenicum Murr.
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黑果枸杞多糖 LRP4-A 的分离和结构表征。
DOI：
10.1016/j.carres.2012.10.013
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发表时间：
2013-01-10
影响因子：
3.1
通讯作者：
Wang, Zhongfu
中科院分区：
文献类型：
--
作者：
Lv, Xiaopeng;Wang, Chengjian;Wang, Zhongfu
从宁夏枸杞果实中分离得到一种复杂的多糖，命名为LRP4-A。并对其结构进行了表征。从平菇果实中分离得到粗多糖LRP。采用热水提取乙醇沉淀法。LRP经阴离子交换层析和凝胶过滤层析，得到LRP4-A水溶性多糖组分。其相对分子质量为1.05×10(5)Da。单糖组成分析表明，LRP4-A主要由鼠李糖、阿拉伯糖、葡萄糖和半乳糖组成，摩尔比为1：7.6：0.5：8.6，并含有少量木糖。采用甲基化分析、部分酸解、红外光谱、核磁共振、电喷雾质谱等一系列分析技术对多糖LRP4-A进行了结构表征。LRP4-A是一种以β-(1->；6)连接的半乳糖为骨架，O-3位部分取代的高度分枝多糖。这些分支由(1->；3)-连接-Gal、(1->；3)-连接-ara、(1->；5)-连接-ara和(1->；2，4)-连接-Rha组成。阿拉伯糖、半乳糖和葡萄糖位于枝条末端。(C)2012爱思唯尔有限公司。保留所有权利。
A complex polysaccharide, termed LRP4-A, was isolated from the fruit of Lycium ruthenicum Murr. and its structure was characterized. The crude polysaccharide LRP was obtained from the fruit of L. ruthenicum Murr. using hot water extraction followed by ethanol precipitation. The water-soluble polysaccharide LRP4-A was purified from LRP by anion-exchange chromatography and gel filtration chromatography. Its molecular weight was 1.05 x 10(5) Da. Monosaccharide composition analysis revealed that LRP4-A mainly consisted of rhamnose, arabinose, glucose, and galactose in the molar ratio of 1:7.6:0.5:8.6, with a trace of xylose. Structure of the polysaccharide LRP4-A was characterized using a series of analytical techniques, including methylation analysis, partial acid hydrolysis, IR, NMR, and ESI-MS. LRP4-A was identified to be a highly branching polysaccharide with a backbone of beta-(1 -> 6)-linked galactose partially substituted at O-3 position. The branches were composed of (1 -> 3)-linked-Gal, (1 -> 3)-linked-Ara, (1 -> 5)-linked-Ara, and (1 -> 2,4)-linked-Rha. Arabinose, galactose, and glucose were located at the termini of the branches. (C) 2012 Elsevier Ltd. All rights reserved.