Endocrine and behavioral effects of airpuff-startle in rats.
Endocrine and behavioral effects of airpuff-startle in rats.
复制标题
吹气惊吓对大鼠的内分泌和行为影响。
DOI：
10.1016/0306-4530(96)00006-6
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发表时间：
1996
影响因子：
3.7
通讯作者：
Plotsky,PM
中科院分区：
文献类型：
--
作者：
Engelmann,M;Thrivikraman,KV;Su,Y;Nemeroff,CB;Montkowski,A;Landgraf,R;Holsboer,F;Plotsky,PM
成年雄性大鼠长期植入套管在颈静脉被用来表征内分泌和行为后果的气胀惊吓。在第一系列实验中，接受三组空气抽吸（每组三次空气抽吸，每次间隔1分钟）的休息动物显示血浆促肾上腺皮质激素（ACTH）和皮质酮水平增加10倍，表明与未处理的对照组（每组n = 5）相比，下丘脑-垂体-肾上腺（HPA）轴显著但中度激活。在第二系列实验中，对防御性退缩范式中焦虑相关行为的监测显示，与未治疗的对照组相比（各n = 10），使用喷气惊吓引起的焦虑显着增加。然而，这种行为效应与血浆激素水平无关，因为在暴露于喷气惊吓和暴露于防御性退缩范式之前和12分钟后立即采集的血液样本显示两组ACTH和皮质酮显著升高。总之，本研究的结果表明，喷气惊吓（1）是激活HPA轴的有效刺激，（2）增加焦虑相关行为，如防御性退缩范式所测量的。此外，我们的研究结果支持这样的假设，即用于测量HPA轴激活的外周内分泌参数不一定与被认为测量焦虑的测试中获得的行为数据相关。
Adult male rats chronically implanted with cannulae in the jugular vein were used to characterize the endocrine and behavioral consequences of airpuff-startle. In the first series of experiments, resting animals subjected to three blocks of airpuff (blocks of three airpuffs each with each block separated by 1 min) showed a 10-fold increase in plasma adrenocorticotropin (ACTH) and corticosterone levels, indicating a significant but moderate activation of the hypothalamo-pituitary-adrenal (HPA) axis when compared with the untreated controls (n = 5 each). In the second series of experiments, monitoring of anxiety-related behavior in the defensive withdrawal paradigm revealed a significant increase in anxiety induced by airpuff-startle application compared with the untreated controls (n = 10 each). This behavioral effect, however, was not correlated with plasma hormone levels, as blood samples taken immediately before and 12 min after exposure to airpuff-startle and exposure to the defensive withdrawal paradigm showed a significant rise in ACTH and corticosterone in both groups. In summary, the findings of the present study indicate that airpuff-startle (1) is a potent stimulus in the activation of the HPA axis, and (2) increases anxiety-related behavior as measured in the defensive withdrawal paradigm. Furthermore, the results of our study support the hypthesis that peripheral endocrine parameters that are used to measure activation of the HPA axis do not necessarily correlate with behavioral data obtained in tests which are thought to measure anxiety.