Stem-loop IV of Tetrahymena telomerase RNA stimulates processivity in trans
Stem-loop IV of Tetrahymena telomerase RNA stimulates processivity in trans
复制标题
DOI：
10.1128/mcb.23.16.5606-5613.2003
复制
发表时间：
2003-08-01
影响因子：
5.3
通讯作者：
Greider, CW
中科院分区：
文献类型：
--
作者：
Mason, DX;Goneska, E;Greider, CW
端粒酶是一种核糖核蛋白酶，负责将端粒添加到染色体末端。端粒短或功能失调可导致细胞生长停滞、凋亡和基因组不稳定。端粒酶利用其RNA亚基复制端粒合成的短模板区域。为了探测四膜虫端粒酶RNA的功能所必需的区域，我们测定了27个循环排列的RNA缺失的端粒酶体外活性和结合的端粒酶逆转录酶催化蛋白亚基。我们发现茎环IV是野生型端粒酶体外活性所必需的，并且当以反式添加时将刺激持续合成能力。
Telomerase is a ribonucleoprotein enzyme responsible for the addition of telomeres onto the ends of chromosomes. Short or dysfunctional telomeres can lead to cell growth arrest, apoptosis, and genomic instability. Telomerase uses its RNA subunit to copy a short template region for telomere synthesis. To probe for regions of Tetrahymena telomerase RNA essential for function, we assayed 27 circularly permuted RNA deletions for telomerase in vitro activity and binding to the telomerase reverse transcriptase catalytic protein subunit. We found that stem-loop IV is required for wild-type telomerase activity in vitro and will stimulate processivity when added in trans.