Surface and Size Effects on Cell Interaction of Gold Nanoparticles with Both Phagocytic and Nonphagocytic Cells
Surface and Size Effects on Cell Interaction of Gold Nanoparticles with Both Phagocytic and Nonphagocytic Cells
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金纳米粒子与吞噬细胞和非吞噬细胞相互作用的表面和尺寸效应
DOI：
10.1021/la401556k
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发表时间：
2013-07-23
期刊：
影响因子：
3.9
通讯作者：
Ji, Jian
中科院分区：
文献类型：
--
作者：
Liu, Xiangsheng;Huang, Nan;Ji, Jian
随着纳米技术的发展及其在生物医学中的应用，纳米粒子与细胞相互作用的研究变得越来越重要。为了了解纳米颗粒的表面和尺寸对细胞相互作用的影响，在吞噬RAW 264.7和非吞噬HepG 2细胞中研究了具有正电荷和负电荷表面的两个系列的金纳米颗粒（AuNPs）的细胞摄取行为，所述两个系列的金纳米颗粒（AuNPs）的尺寸范围从类似于16 nm到类似于58 nm。通过ICP-MS定量AuNPs的内化，并通过TEM分析评估NPs的细胞内命运。结果表明，表面带正电荷的金纳米粒子在非吞噬细胞HepG 2中具有更强的细胞内化能力。然而，当在吞噬RAW 264.7细胞中时，带负电荷的AuNPs的摄取程度与带正电荷的AuNPs的摄取程度相似。在所测试的尺寸范围中，直径接近40 nm的带负电荷的AuNPs在两种细胞中具有最高的摄取，而带正电荷的AuNPs没有显示出一定的趋势。细胞内透射电镜分析表明，不同的细胞中的金纳米粒子的不同命运，其中带正电荷和负电荷的金纳米粒子主要被困在溶酶体中的HepG 2细胞，但其中许多定位在吞噬体时，在RAW 264.7细胞。MTT法和LDH法检测了纳米金的细胞毒性，结果表明纳米金的细胞毒性除与纳米金的表面和大小有关外，还与所检测的细胞类型有关。这表明纳米颗粒与细胞之间的细胞相互作用不仅受表面和尺寸因素的影响，而且强烈依赖于细胞类型。
With the development of nanotechnology and its application in biomedicine, studies on the interaction between nanoparticles and cells have become increasingly important. To understand the surface and size effects on cell interaction of nanoparticles, the cellular uptake behaviors of two series of gold nanoparticles (AuNPs) with both positively and negatively charged surfaces and sizes range from similar to 16 to similar to 58 nm were investigated in both phagocytic RAW 264.7 and nonphagocytic HepG2 cells. The internalization of AuNPs was quantified by ICP-MS, and the intracellular fate of NPs was evaluated by TEM analysis. The results showed that the AuNPs with positive surface charge have much higher cell internalization ability than those with negative surface charge in nonphagocytic HepG2 cells. However, the uptake extent of negatively charged AuNPs was similar with that of the positively charged AuNPs when in phagocytic RAW 264.7 cells. Among the tested size range, negatively charged AuNPs with a diameter of similar to 40 nm had the highest uptake in both cells, while the positively charged AuNPs did not show a certain tendency. Intracellular TEM analysis demonstrated the different fate of AuNPs in different cells, where both the positively and negatively charged AuNPs were mainly trapped in the lysosomes in HepG2 cells, but many of them were localized in phagosomes when in RAW 264.7 cells. Cytotoxicity of these AuNPs was tested by both MTT and LDH assays, which suggested NP's toxicity is closely related to the tested cell types besides the surface and size of NPs. It demonstrates that cell interaction between nanopartides and cells is not only affected by surface and size factors but also strongly depends on cell types.