Carbon nanotube-assisted enhancement of surface plasmon resonance signal
Carbon nanotube-assisted enhancement of surface plasmon resonance signal
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DOI：
10.1016/j.ab.2010.09.026
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发表时间：
2011-01-15
影响因子：
2.9
通讯作者：
Chung, Bong Hyun
中科院分区：
文献类型：
--
作者：
Lee, Eun Gyo;Park, Kyung Mi;Chung, Bong Hyun
我们描述了一种利用抗体-碳纳米管（CNT）偶联物在基于表面等离子体共振（SPR）的免疫分析中放大生物传感信号的方法。以SPR生物传感器为模型系统，采用夹心免疫法检测人促红细胞生成素（EPO）和人粒细胞巨噬细胞集落刺激因子（GM-CSF）。为了扩增SPR信号，将碳纳米管与多克隆抗体偶联，然后与偶联目标蛋白的抗体反应。这种扩增策略增加了免疫测定的动态范围，提高了检测灵敏度。SPR免疫分析结合碳纳米管辅助信号放大方法，为EPO和GM-CSF提供了超过四个数量级的宽动态范围（0.1-1000 ng/ml）。碳纳米管扩增方法有望实现皮克图水平的检测和多种蛋白质的宽动态检测范围，使其能够为生物制药生产的发展提供强大的分析工具。(c) 2010爱思唯尔公司版权所有。
We describe a method of amplifying the biosensing signal in surface plasmon resonance (SPR)-based immunoassays using an antibody-carbon nanotube (CNT) conjugate. As a model system, human erythropoietin (EPO) and human granulocyte macrophage colony-stimulating factor (GM-CSF) were detected by sandwich-type immunoassays using an SPR biosensor. For the amplification of the SPR signal, the CNT was conjugated with a polyclonal antibody, and then the conjugates were reacted with antibodies coupled with the target proteins. This amplification strategy increases the dynamic range of the immunoassays and enhances the detection sensitivity. The SPR immunoassays, combined with the CNT-assisted signal amplification method, provided a wide dynamic range over four orders of magnitude for both EPO and GM-CSF (0.1-1000 ng/ml). The CNT amplification method is expected to realize the detection of picogram levels and a wide dynamic detection range of multiple proteins, enabling it to offer a robust analysis tool for the development of biopharmaceutical production. (c) 2010 Elsevier Inc. All rights reserved.