A facile method to prepare a versatile surface coating with fibrinolytic activity, vascular cell selectivity and antibacterial properties
A facile method to prepare a versatile surface coating with fibrinolytic activity, vascular cell selectivity and antibacterial properties
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一种制备具有纤溶活性、血管细胞选择性和抗菌特性的多功能表面涂层的简便方法
DOI：
10.1016/j.colsurfb.2018.03.047
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chen Hong
中科院分区：
文献类型：
--
作者：
Jin Sheng;Gu Hao;Chen Xianshuang;Liu Xiaoli;Zhan Wenjun;Wei Ting;Sun Xuebo;Ren Chuanlu;Chen Hong
对于血液接触装置来说，在与血液接触的表面上形成凝块和血栓仍然是最严重的问题。尽管在血液相容性材料的开发上持续了多年的努力，但开发多功能材料来实现血管细胞的选择性（有利于快速内皮化，同时抑制平滑肌细胞的增殖）和改善血液相容性仍然是人们非常感兴趣的。此外，生物材料相关感染也会导致生物医学植入物和设备的失效。然而，设计多功能材料仍然是一项具有挑战性的任务，因为它们的一种功能通常会因引入另一种功能而受到损害。在本工作中，金衬底首先被一层一层（LbL）地沉积在多层聚电解质薄膜上，该薄膜含有壳聚糖（带正电荷）和4-乙烯基苯磺酸钠（SS）的共聚物，以及“客体”金刚烷单体1-金刚烷-1-甲基丙烯酸甲酯（P(SS-co- ada)，带负电荷），通过静电相互作用称为Au-LbL。壳聚糖和P（SS-co-Ada）分别具有抗细菌和类肝素特性。然后，通过金刚烷残基与CD-L之间的主客体相互作用，将含有7个赖氨酸配体的“宿主”β-环糊精衍生物（CD-L）固定在Au-LbL表面，称为Au-LbL/CD-L。最后，开发了一种具有纤维蛋白溶解活性（新生凝块的裂解）、血管细胞选择性和抗菌性能的多功能表面涂层。
Clot and thrombus formation on surfaces that come into contact with blood is still the most serious problem for blood contacting devices. Despite many years of continuous efforts in developing hemocompatible materials, it is still of great interest to develop multifunctional materials to enable vascular cell selectivity (to favor rapid endothelialization while inhibiting smooth muscle cell proliferation) and improve hemocompatibility. In addition, biomaterial-associated infections also cause the failure of biomedical implants and devices. However, it remains a challenging task to design materials that are multifunctional, since one of their functions will usually be compromised by the introduction of another function. In the present work, the gold substrate was first layer-by-layer (LbL) deposited with a multilayered polyelectrolyte film containing chitosan (positively charged) and a copolymer of sodium 4-vinylbenzenesulfonate (SS) and the “guest” adamantane monomer 1-adamantan-1-ylmethyl methacrylate (P(SS-co-Ada), negatively charged) via electro-static interactions, referred to as Au-LbL. The chitosan and P(SS-co-Ada) were intended to provide, respectively, resistance to bacteria and heparin-like properties. Then, “host” β-cyclodextrin derivatives bearing seven lysine ligands (CD-L) were immobilized on the Au-LbL surface by host-guest interactions between adamantane residues and CD-L, referred to as Au-LbL/CD-L. Finally, a versatile surface coating with fibrinolytic activity (lysis of nascent clots), vascular cell selectivity and antibacterial properties was developed.