Actomyosin contractility in olfactory placode neurons opens the skin epithelium to form the zebrafish nostril.
Actomyosin contractility in olfactory placode neurons opens the skin epithelium to form the zebrafish nostril.
复制标题
嗅板神经元中的肌动球蛋白收缩性打开皮肤上皮形成斑马鱼鼻孔。
DOI：
10.1016/j.devcel.2023.02.001
复制
发表时间：
2023
影响因子：
11.8
通讯作者：
Breau,MarieAnne
中科院分区：
文献类型：
--
作者：
Baraban,Marion;GordilloPi,Clara;Bonnet,Isabelle;Gilles,Jean-François;Lejeune,Camille;Cabrera,Mélody;Tep,Florian;Breau,MarieAnne
尽管上皮具有屏障功能，但在形态发生过程中，上皮可能局部失去完整性，从而产生生理开口。驱动这些上皮断裂形成的机制才刚刚开始被研究。在这里，我们研究了斑马鱼鼻孔（嗅觉孔）的形成，它在皮肤上皮中打开，使嗅觉神经元暴露于外部气味线索。结合实时成像、药物治疗、激光消融和组织特异性功能扰动，我们描述了嗅觉基板神经元和皮肤之间的机械相互作用，这在孔的形成中起着至关重要的作用：神经元以肌动球蛋白依赖的方式拉上覆盖的皮肤细胞，结合皮肤上皮的局部重组，触发孔的开放。这项工作确定了破坏上皮片的原始机制，其中邻近的一组细胞机械地协助上皮诱导其局部破裂。
Despite their barrier function, epithelia can locally lose their integrity to create physiological openings during morphogenesis. The mechanisms driving the formation of these epithelial breaks are only starting to be investigated. Here, we study the formation of the zebrafish nostril (the olfactory orifice), which opens in the skin epithelium to expose the olfactory neurons to external odorant cues. Combining live imaging, drug treatments, laser ablation, and tissue-specific functional perturbations, we characterize a mechanical interplay between olfactory placode neurons and the skin, which plays a crucial role in the formation of the orifice: the neurons pull on the overlying skin cells in an actomyosin-dependent manner which, in combination with a local reorganization of the skin epithelium, triggers the opening of the orifice. This work identifies an original mechanism to break an epithelial sheet, in which an adjacent group of cells mechanically assists the epithelium to induce its local rupture.