Cloning and characterization of rat C3a receptor: differential expression of rat C3a and C5a receptors by LPS stimulation.
Cloning and characterization of rat C3a receptor: differential expression of rat C3a and C5a receptors by LPS stimulation.
复制标题
大鼠 C3a 受体的克隆和表征：LPS 刺激下大鼠 C3a 和 C5a 受体的差异表达。
DOI：
10.1006/bbrc.1997.8034
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Hugli,TE
中科院分区：
文献类型：
--
作者：
Fukuoka,Y;Ember,JA;Hugli,TE
补体激活过程中产生的过敏毒素 C3a 是已知介导各种炎症反应的因子。人类 C3a 受体 (C3aR) 最近被克隆并鉴定为 G 蛋白偶联受体家族的成员。 C3aR 的特征是七个跨膜结构域，包括一个大的第二胞外环，这似乎是该受体的独特特征。在这里，我们报告了大鼠 C3aR 克隆的分离，并根据 C3a 与稳定转染细胞的结合分析，确认分离的 cDNA 编码大鼠 C3aR。大鼠 C3aR 的 Northern 印迹分析揭示了在各种组织中的表达，与人 C3aR 相似，但与大鼠 C5aR 不同。我们发现注射LPS后大鼠各组织中C3aR的表达没有显着增加，而大鼠C5aR的表达大大增加。这些结果表明 C3aR 和 C5aR 的表达在大鼠细胞和组织中受到独立调节。
The anaphylatoxin C3a, generated during complement activation, is a factor known to mediate various inflammatory reactions. The human C3a receptor (C3aR) was recently cloned and identified to be a member of the G-protein-coupled receptor family. C3aR is characterized by seven transmembrane domains including a large second extracellular loop that appears to be a unique feature of this receptor. Here we report the isolation of the rat C3aR clone and confirm that the isolated cDNA coded for rat C3aR based on C3a binding analysis to stably transfected cells. Northern blot analysis of rat C3aR revealed expression in various tissues, similar to that of human C3aR but dissimilar to rat C5aR. We found that expression of rat C3aR in various tissues did not increase significantly after LPS injection, whereas rat C5aR expression is greatly increased. These results suggest that expression of C3aR and C5aR is independently regulated in rat cells and tissues.