Downregulation of cylindromatosis gene, CYLD, confers a growth advantage on malignant melanoma cells while negatively regulating their migration activity
Downregulation of cylindromatosis gene, CYLD, confers a growth advantage on malignant melanoma cells while negatively regulating their migration activity
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DOI：
10.3892/ijo.2012.1424
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发表时间：
2012-07-01
影响因子：
5.2
通讯作者：
Maesawa, Chihaya
中科院分区：
文献类型：
--
作者：
Ishikawa, Yuichi;Tsunoda, Kanako;Maesawa, Chihaya
圆柱瘤病基因（CYLD）编码一种去泛素酶，该酶最初被鉴定为肿瘤抑制因子，最近被研究与多种正常生理过程有关。与其细胞增殖活性相反，CYLD蛋白对细胞迁移的影响一直是一个有争议的问题。我们研究了CYLD-siRNA对恶性黑色素瘤细胞迁移活性的影响。CYLD mRNA/蛋白的表达在8个恶性黑色素瘤细胞系中的6个中低于3组原代培养的正常人表皮黑色素细胞。敲低CYLD显著增加了两种黑色素瘤细胞系的增殖活性（p
The cylindromatosis gene (CYLD) encodes a deubiq-uitinase that was initially identified as a tumor suppressor and has recently been investigated in connection with a variety of normal physiological processes. In contrast to its cell-proliferative activity, the effect of CYLD protein on cell migration has been a matter of debate. We investigated the effect of CYLD-siRNA on the migration activity of malignant melanoma cells. Expression of CYLD mRNA/protein was lower in 6 of 8 malignant melanoma cell lines than in 3 sets of primary-cultured normal human epidermal melanocytes. Knockdown of CYLD significantly increased the proliferation activities of two melanoma cell lines (p