Complex coupled metabolic and prokaryotic community responses to increasing temperatures in anaerobic marine sediments: critical temperatures and substrate changes.
Complex coupled metabolic and prokaryotic community responses to increasing temperatures in anaerobic marine sediments: critical temperatures and substrate changes.
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DOI：
10.1093/femsec/fiv084
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发表时间：
2015-08
影响因子：
4.2
通讯作者：
Parkes RJ
中科院分区：
文献类型：
--
作者：
Roussel EG;Cragg BA;Webster G;Sass H;Tang X;Williams AS;Gorra R;Weightman AJ;Parkes RJ
在泥浆中研究了温度（0 - 80 ° C）对海洋沉积物（潮滩）中厌氧生物地球化学过程和原核生物群落的影响，研究时间长达100天。温度对生物地球化学和原核生物有非线性影响，在很小的温度间隔内变化迅速。有些活动（如甲烷生成）在很大的温度范围内（10至80 ° C）有多个"窗口"。其他的，包括乙酸盐氧化，在一个温度区域内具有最大活性，该温度区域因电子受体而异[金属氧化物（高达34 ° C）和硫酸盐（高达50 ° C）]。在43 ° C的临界温度下，硫酸盐还原的底物从主要是乙酸盐和H2改变，沿着活化能和硫酸盐还原菌的类型发生变化。高于1043 ° C，甲胺代谢随着产甲烷菌类型的变化和乙酸盐浓度的增加（> 1 mM）而停止。未培养的古细菌的丰度，深海沉积物的特点（如MBGD Euryarchaeota，'Bathyarchaeota'）的变化，表明他们可能的代谢活动和温度范围。细菌细胞的数量始终高于古细菌细胞，并且在1015 ° C以上都有所下降。底物的添加刺激了活性，拓宽了某些活性温度范围（产甲烷），并增加了细菌（× 10）的细胞数量。因此，额外的有机物输入与气候有关的富营养化可能会放大温度升高对沉积物地球化学的影响。温度升高对海洋沉积物中的生物地球化学和原核生物产生非线性影响，在与气候变化和深层生物圈有关的小温度间隔内迅速变化。温度升高对海洋沉积物中的生物地球化学和原核生物产生非线性影响，在与气候变化和深层生物圈有关的小温度间隔内迅速变化。
The impact of temperature (0–80°C) on anaerobic biogeochemical processes and prokaryotic communities in marine sediments (tidal flat) was investigated in slurries for up to 100 days. Temperature had a non-linear effect on biogeochemistry and prokaryotes with rapid changes over small temperature intervals. Some activities (e.g. methanogenesis) had multiple ‘windows’ within a large temperature range (∼10 to 80°C). Others, including acetate oxidation, had maximum activities within a temperature zone, which varied with electron acceptor [metal oxide (up to ∼34°C) and sulphate (up to ∼50°C)]. Substrates for sulphate reduction changed from predominantly acetate below, and H2 above, a 43°C critical temperature, along with changes in activation energies and types of sulphate-reducing Bacteria. Above ∼43°C, methylamine metabolism ceased with changes in methanogen types and increased acetate concentrations (>1 mM). Abundances of uncultured Archaea, characteristic of deep marine sediments (e.g. MBGD Euryarchaeota, ‘Bathyarchaeota’) changed, indicating their possible metabolic activity and temperature range. Bacterial cell numbers were consistently higher than archaeal cells and both decreased above ∼15°C. Substrate addition stimulated activities, widened some activity temperature ranges (methanogenesis) and increased bacterial (×10) more than archaeal cell numbers. Hence, additional organic matter input from climate-related eutrophication may amplify the impact of temperature increases on sedimentary biogeochemistry. Temperature increases had a non-linear effect on biogeochemistry and prokaryotes in marine sediments, with rapid changes over small temperature intervals which are relevant to climate change and the deep biosphere. Temperature increases had a non-linear effect on biogeochemistry and prokaryotes in marine sediments, with rapid changes over small temperature intervals which are relevant to climate change and the deep biosphere.