Holographic glucose sensors
Holographic glucose sensors
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DOI：
10.1016/j.bios.2004.07.005
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发表时间：
2005-02-15
影响因子：
12.6
通讯作者：
Lowe, CR
中科院分区：
文献类型：
--
作者：
Kabilan, S;Marshall, AJ;Lowe, CR
研制了一种新型的能够检测葡萄糖浓度动态变化的全息传感器系统。全息图记录在含有苯基硼酸衍生物的生物相容水凝胶基质中。在结合葡萄糖时，随着水凝胶的膨胀，全息图的颜色红移到更长的波长，这种颜色变化被用来量化葡萄糖浓度。然而，苯基硼酸是非选择性的，可以与多种顺式二醇结合。在血液中，葡萄糖是唯一在高浓度下发现的游离糖，而其他糖通常是糖蛋白和大分子结构的一部分。虽然糖蛋白已被证明对传感器没有影响，但苯基硼酸比葡萄糖更容易与乳酸结合。我们设计了两种聚合物水凝胶体系，以提高传感器对葡萄糖和乳酸的选择性。第一个涉及使用高浓度的3-丙烯酰胺基苯基硼酸(3-APB)，而第二个系统使用2-丙烯酰胺基-5-氟苯基硼酸(5-F-2-MAPB)。在生理pH和离子强度条件下，这两种体系对葡萄糖的选择性均高于乳酸，可作为检测生理液体中葡萄糖的选择性全息传感器。(C)2004年，爱思唯尔出版。
A novel holographic sensor system capable of detecting dynamic changes in glucose concentration has been developed. The hologram is recorded within a bio-compatible hydrogel matrix containing phenylboronic acid derivatives. On binding glucose, the colour of the hologram red-shifts to longer wavelengths as the hydrogel expands and this colour change is used to quantify glucose concentration. However, phenylboronic acids are non-selective and bind a wide variety of cis-diols. In blood, glucose is the only sugar found free at high concentration, whilst other sugars are typically found as part of glycoproteins and macromolecular structures. Although glycoproteins have been shown to have no effect on the sensor, phenylboronic acids can bind lactate much more readily than glucose. We have designed two polymer hydrogel systems to increase the selectivity of the sensor for glucose over lactate. The first involved the use of high concentrations of 3-acrylamidophenylboronic acid (3-APB) whilst the second system utilised 2-acrylamido-5-fluorophenylboronic acid (5-F-2-MAPB). Both systems displayed an increased selectivity to glucose over lactate at physiological pH and ionic strength and could be deployed as selective holographic sensors for glucose detection in physiological fluids. (C) 2004 Published by Elsevier B.V.