Basidioascus undulatus: genome, origins, and sexuality.
Basidioascus undulatus: genome, origins, and sexuality.
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DOI：
10.5598/imafungus.2015.06.01.14
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发表时间：
2015-06
期刊：
影响因子：
5.4
通讯作者：
Seifert KA
中科院分区：
文献类型：
--
作者：
Nguyen HD;Chabot D;Hirooka Y;Roberson RW;Seifert KA
波纹担子菌（Basidiococcus undulatus）是一种土壤担子菌，属于双生担子目（Geminibasidiales）。该目的分类地位尚不清楚，因为最初它只是暂时被分类在Wallemiomycetes纲中。双子担子菌目中的真菌有一个定义模糊的性周期。本研究对B的基因组进行了测序。以深入了解其性别和进化起源。组装的基因组草图大小约为32 Mb，具有24 X的中值核苷酸覆盖率，并且包含6123个预测基因。先前对B的形态学描述。undulatus依赖于对假定的性结构的解释。在这项研究中，核染色和共聚焦显微镜显示减数分裂发生在担子和基因组分析证实了基因的存在参与减数分裂和交配。利用从基因组信息中提取的35个蛋白质编码基因，通过基因组和分子年代学分析，证实了B。波生菌确实属于与瓦耳菌属有远亲关系的一个谱系，同时在伞菌亚门中保持着基础地位。这些结果，结合中隔孔形态的差异，使我们移动的目的Geminibasidiales的Wallemiomycetes和进入新的类Geminibasidiomycetes cl。最后，对伞菌亚门的概念进行了修正，使之包括瓦氏菌纲和双子担子菌纲。
Basidioascus undulatus is a soil basidiomycete belonging to the order Geminibasidiales. The taxonomic status of the order was unclear as originally it was only tentatively classified in the class Wallemiomycetes. The fungi in Geminibasidiales have an ambiguously defined sexual cycle. In this study, we sequenced the genome of B. undulatus to gain insights into its sexuality and evolutionary origins. The assembled genome draft was approximately 32 Mb in size, had a median nucleotide coverage of 24X, and contained 6123 predicted genes. Previous morphological descriptions of B. undulatus relied on interpretation of putative sexual structures. In this study, nuclear staining and confocal microscopy showed meiosis occurring in basidia and genome analysis confirmed the existence of genes involved in meiosis and mating. Using 35 protein-coding genes extracted from genomic information, phylogenomic and molecular dating analyses confirmed that B. undulatus indeed belongs to a lineage distantly related to Wallemia while retaining a basal position in Agaricomycotina. These results, combined with differences in septal pore morphology, led us to move the order Geminibasidiales out of the Wallemiomycetes and into the new class Geminibasidiomycetes cl. nov. Finally, the concept of Agaricomycotina is emended to include both Wallemiomycetes and Geminibasidiomycetes.