Structural Identification of Theaflavin Trigallate and Tetragallate from Black Tea Using Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry
Structural Identification of Theaflavin Trigallate and Tetragallate from Black Tea Using Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry
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DOI：
10.1021/jf303749z
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发表时间：
2012-10-31
影响因子：
6.1
通讯作者：
Sang, Shengmin
中科院分区：
文献类型：
--
作者：
Chen, Huadong;Shurlknight, Kelly;Sang, Shengmin
红茶含有两种主要的色素，茶黄素和茶红素。这些多酚具有一定的健康益处，包括预防心脏病和癌症。茶红素是红茶中最丰富的色素，多年来，阐明和表征茶红素的结构一直是一个挑战。因此，必须对红茶多酚进行进一步的研究，以努力解决这一争议。本研究采用液相色谱/电喷雾电离质谱分析方法，发现红茶提取物中含有三酸茶黄素和四酸茶黄素。这些结构通过MSn （n = 1-4）谱分析和产物离子的MS/MS谱与正品(-)-表没食子儿茶素-3-没食子酸酯、(-)-表儿茶素-3-没食子酸酯和茶黄素-3,3'-双二酸酯的MS/MS谱比较得到证实。据我们所知，这是第一个证实红茶中存在三酸茶黄素和四酸茶黄素的报告。
Black tea contains two major pigments, theaflavins and thearubigins. These polyphenols have been associated with certain health benefits including prevention of heart disease and cancer. Elucidating and characterizing the structural aspects of thearubigins, the most abundant pigment in black tea, has been a challenge for many years. Therefore further studies of black tea polyphenols must be conducted in effort to solve this thearubigin dispute. In the present study, black tea extract was found to possess theaflavin trigallate and tetragallate by means of liquid chromatography/electrospray ionization mass spectrometry. These structures were confirmed by analysis of the MSn (n = 1-4) spectra and comparison of the MS/MS spectra of the product ions to the MS/MS spectra of authentic (-)-epigallocatechin-3-gallate, (-)-epicatechin-3-gallate and theaflavin-3,3'-digallate. To our knowledge, this is the first report to confirm the presence of theaflavin trigallate and tetragallate in black tea.