Interleukin 2 receptors on human B cells. Implications for the role of interleukin 2 in human B cell function.
Interleukin 2 receptors on human B cells. Implications for the role of interleukin 2 in human B cell function.
复制标题
DOI：
10.1084/jem.161.1.181
复制
发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
Fauci AS
中科院分区：
文献类型：
--
作者：
Muraguchi A;Kehrl JH;Longo DL;Volkman DJ;Smith KA;Fauci AS
在本研究中，我们检测了正常人 B 细胞以及已建立的 B 细胞系上白细胞介素 2 (IL-2) 受体的表达。抗 Tac 单克隆抗体不与新鲜分离的正常人 B 细胞结合。出乎意料的是，通过抗mu抗体、佛波醇肉豆蔻酸酯或金黄色葡萄球菌Cowan I (SAC)适当激活正常B细胞，在激活的B细胞上诱导出Tac抗原。抗 Tac 抗体与被人 T 细胞白血病病毒 (HTLV) 感染的两种 B 细胞系表现出一致的反应性，并且与从正常成年供体建立的八个 Epstein-Barr 病毒转化 B 细胞系中的两种表现出一定的反应性。免疫沉淀分析显示，来自植物血凝素刺激的正常 T 细胞母细胞和正常活化的 B 细胞以及克隆的 B 细胞系的抗 Tac 抗体可以沉淀出大小相似、分子量为 50,000-60,000 的抗原。 IL-2 对正常活化 B 细胞和克隆 B 细胞 (HS1) 的结合测定表明，与植物血凝素刺激的正常 T 细胞母细胞相比，B 细胞具有明显更少的位点和更低的亲和力 IL-2 受体。最后，通过将 B 细胞与重组 IL-2 一起孵育来检查 B 细胞上 IL-2 受体的生物学特性。结果发现，中等浓度的IL-2可显着增强SAC激活的正常B细胞的增殖和分化。这些结果表明，正常B细胞可能表达功能性IL-2受体或密切相关的蛋白质，因此IL-2可能在B细胞功能的调节中发挥重要作用。
In the present study, we examined the expression of interleukin 2 (IL- 2) receptors on normal human B cells as well as established B cell lines. Anti-Tac monoclonal antibody did not bind to freshly separated normal human B cells. Unexpectedly, with the appropriate activation of the normal B cells by anti-mu antibody, phorbol myristate acetate, or Staphylococcus aureus Cowan I (SAC), Tac antigen was induced on the activated B cells. Anti-Tac antibody showed consistent reactivity with two B cell lines that were infected by human T cell leukemia virus (HTLV) and some reactivity with two out of eight Epstein-Barr virus- transformed B cell lines established from normal adult donors. Immunoprecipitation analysis revealed that antigens of similar size with a molecular weight of 50,000-60,000 can be precipitated with anti- Tac antibody from phytohemagglutinin-stimulated normal T cell blasts and normal activated B cells, as well as a cloned B cell line. Binding assays of IL-2 on normal activated B cells and on the cloned B cell (HS1) revealed that B cells have significantly fewer sites and lower- affinity IL-2 receptors compared with phytohemagglutinin-stimulated normal T cell blasts. Finally, biological properties of the IL-2 receptor on B cells were examined by incubating B cells with recombinant IL-2. It was found that moderate concentrations of IL-2 induce significant enhancement of proliferation and differentiation in SAC-activated normal B cells. These results suggest that normal B cells may express functional IL-2 receptors or closely related proteins and thus IL-2 may play a significant role in the modulation of B cell function.