HERBICIDE-RESISTANT INDICA RICE PLANTS FROM IRRI BREEDING LINE IR72 AFTER PEG-MEDIATED TRANSFORMATION OF PROTOPLASTS
HERBICIDE-RESISTANT INDICA RICE PLANTS FROM IRRI BREEDING LINE IR72 AFTER PEG-MEDIATED TRANSFORMATION OF PROTOPLASTS
复制标题
DOI：
10.1007/bf00046447
复制
发表时间：
1992-11-01
影响因子：
5.1
通讯作者：
POTRYKUS, I
中科院分区：
文献类型：
--
作者：
DATTA, SK;DATTA, K;POTRYKUS, I
商业上重要的籼稻栽培品种Oryza sativa cv.已经使用直接基因转移至原生质体来转化IR 72。PEG介导的转化用两种质粒构建体进行，所述质粒构建体含有赋予对潮霉素（Hg）的抗性的CaMV 35 S启动子/HPH嵌合基因或赋予对含有膦丝菌素（PPT）的商业除草剂（Basta）的抗性的CaMV 35 S启动子/BAR嵌合基因。通过原生质体筛选，我们共获得Hg（r）IR 72株92株，PPT（r）IR 72株170株。31株汞（r）植物和70株PPT（r）植物正在温室中生长至成熟。Southern杂交和酶切分析结果表明，转基因植物已稳定整合到宿主基因组中并得到表达。转基因植物表现出对高剂量的PPT商业制剂的完全抗性。
The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or a CaMV 35S promoter/BAR chimaeric gene conferring resistance to a commercial herbicide (Basta) containing phosphinothricin (PPT). We have obtained so far 92 Hg(r) and 170 PPT(r) IR72 plants from protoplasts through selection. 31 Hg(r) and 70 PPT(r) plants are being grown in the greenhouse to maturity. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed. Transgenic plants showed complete resistance to high doses of the commercial formulations of PPT.