3D-printed chitosan-based scaffolds: An in vitro study of human skin cell growth and an in-vivo wound healing evaluation in experimental diabetes in rats
3D-printed chitosan-based scaffolds: An in vitro study of human skin cell growth and an in-vivo wound healing evaluation in experimental diabetes in rats
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DOI：
10.1016/j.carbpol.2018.07.057
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发表时间：
2018-11-01
影响因子：
11.2
通讯作者：
McConnell, Michelle
中科院分区：
文献类型：
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作者：
Intini, Claudio;Elviri, Lisa;McConnell, Michelle
介绍并讨论了用于皮肤组织再生的多孔3D打印壳聚糖（CH）支架的制造及其在生物相容性，细胞相容性和对人成纤维细胞（Nhdf）和角质形成细胞（HaCaT）的毒性方面的行为。培养20天和35天后获得的3D细胞培养物显示出显著的体外定性和定量细胞生长，如通过中性红染色和MTT测定所测量的，并通过扫描电子显微镜照片所证实的。在3D支架上35天后，当Nhdf和HaCaT细胞一起接种时，获得最佳细胞生长，填充支架中的孔。观察到由大量成纤维细胞和角质形成细胞共同生长组成的早期皮肤样层。3D打印支架在链脲佐菌素诱导的糖尿病大鼠伤口愈合中的测试表明，3D打印支架在商业贴片和自发愈合方面提高了修复组织的质量。
The fabrication of porous 3D printed chitosan (CH) scaffolds for skin tissue regeneration and their behavior in terms of biocompatibility, cytocompatibility and toxicity toward human fibroblasts (Nhdf) and keratinocytes (HaCaT), are presented and discussed. 3D cell cultures achieved after 20 and 35 days of incubation showed significant in vitro qualitative and quantitative cell growth as measured by neutral red staining and MTT assays and confirmed by scanning electron microphotographs. The best cell growth was obtained after 35 days on 3D scaffolds when the Nhdf and HaCaT cells, seeded together, filled the pores in the scaffolds. An early skin-like layer consisting of a mass of fibroblast and keratinocyte cells growing together was observed. The tests of 3D printed scaffolds in wound healing carried out on streptozotocin-induced diabetic rats demonstrate that 3D printed scaffolds improve the quality of the restored tissue with respect to both commercial patch and spontaneous healing.