SOLUBILIZATION OF BACTERIAL-CELLS IN ORGANIC-SOLVENTS VIA REVERSE MICELLES
SOLUBILIZATION OF BACTERIAL-CELLS IN ORGANIC-SOLVENTS VIA REVERSE MICELLES
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DOI：
10.1016/s0006-291x(85)80030-9
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
MEUSSDOERFFER, F
中科院分区：
文献类型：
--
作者：
HARING, G;LUISI, PL;MEUSSDOERFFER, F
反胶束体系中含有吐温85和水的异丙基棕榈酸酯的开发，允许细菌的均匀的有机溶液的形式增溶。通过光学显微镜证实溶液中细菌的存在。溶解后立即观察到分离的细菌细胞，其通过老化倾向于形成较大的聚集体。大肠杆菌的细胞在该系统中保持存活至少1天，并保留β-半乳糖苷酶活性持续甚至更长的时间，如x-gal的水解所示。乙酸钙不动杆菌的烷烃降解菌株的细胞在该系统中保持存活数天。
A reverse micellar system containing Tween 85 and water in isopropylpalmitate was developed which permitted the solubilization of bacteria in the form of homogenous organic solutions. The presence of the bacteria in solution was demonstrated by light microscopy. Immediately after solubilization, isolated bacterial cells were observed, which by aging tend to form larger aggregates. Cells of Escherichia coli remained viable in this system for at least 1 day and retained .beta.-galactosidase activity for an even longer period as indicated by the hydrolysis of x-gal. Cells of an alkane-degrading strain of Acinetobacter calcoaceticus remained viable in the system for several days.