Transcriptome analysis reveals gene expression changes of the fat body of silkworm (Bombyx mori L.) in response to selenium treatment
Transcriptome analysis reveals gene expression changes of the fat body of silkworm (Bombyx mori L.) in response to selenium treatment
复制标题
转录组分析揭示家蚕（Bombyx mori L.）脂肪体响应硒处理的基因表达变化
DOI：
10.1016/j.chemosphere.2019.125660
复制
发表时间：
2020-04-01
期刊：
影响因子：
8.8
通讯作者：
Wei, Zhao-Jun
中科院分区：
文献类型：
--
作者：
Jiang, Li;Peng, Li-Li;Wei, Zhao-Jun
采用比较转录组分析方法，研究了不同浓度（50 μ M和200 μ M）硒处理后家蚕脂肪体基因表达的变化。在50 μ M和200 μ M硒处理的家蚕脂肪体中，分别鉴定出912个差异表达基因（DEG）（371个上调，541个下调）和1420个差异表达基因（DEG）（1078个上调，342个下调）。50 μ M组DEGs主要富集于过氧化物酶体途径和脂肪酸代谢途径，其次与抗氧化防御和营养调节有关。200 μ M硒处理后，DEGs主要定位于甘油脂代谢和花生四烯酸代谢途径，进一步编码解毒相关基因。此外，已经选择了来自这些途径的32个候选DEG来确认RNA-seq数据。其中14个基因在50 μ M硒处理组中表达上调（200 μ M硒处理组中仅3个基因表达上调），这些基因与脂质代谢和抗氧化防御有关; 13个基因在200 μ M硒处理组中表达上调（50 μ M硒处理组中仅2个基因表达上调），这些基因与解毒有关。这些变化表明，低浓度的硒能调节家蚕的营养，促进抗氧化途径，而高浓度的硒能促进家蚕的解毒作用。这些结果有助于了解硒在家蚕等昆虫体内的作用和解毒机制。(C)2019爱思唯尔有限公司版权所有。
A comparative transcriptome analysis was conducted to investigate the gene expression changes in the fat body of silkworm after treatment with different concentrations (50 mu M and 200 mu M) of selenium (Se). 912 differential expression genes (DEGs) (371 up-regulated and 541 down-regulated) and 1420 DEGs (1078 up-regulated and 342 down-regulated) were identified in silkworm fat body treated with 50 mu M and 200 mu M of Se, respectively. In case of 50 mu M group, DEGs were mainly enriched in the peroxisome pathway and fatty acid metabolism pathway, and later were associated with antioxidant defense and nutrition regulation. After 200 mu M Se-treatment, DEGs were mainly located in the glycerolipid metabolism and arachidonic acid metabolism pathways, which further encoded detoxification related genes. Furthermore, 32 candidate DEGs from these pathways had been selected to confirm the RNA-seq data. Among these DEGs, 14 genes were up-regulated in the 50 mu M Se-treated group (only three genes in the 200 mu M Se-treated group) which were involved in lipid metabolism and antioxidant defense, and 13 upregulated genes (only two genes were up-regulated in the 50 mu M Se-treated group) were involved in detoxification of the 200 mu M Se-treated group. These changes showed that lower concentration of Se could regulate the nutrition and promote antioxidation pathways; whereas, high levels of Se promoted the detoxification of silkworm. These findings can be helpful to understand the possible mechanisms of Se action and detoxification in silkworm and other insects. (C) 2019 Elsevier Ltd. All rights reserved.