Enhanced Universal Quantification of Biomolecules Using Element MS and Generic Standards: Application to Intact Protein and Phosphoprotein Determination
Enhanced Universal Quantification of Biomolecules Using Element MS and Generic Standards: Application to Intact Protein and Phosphoprotein Determination
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DOI：
10.1021/acs.analchem.8b04731
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发表时间：
2019-01-01
影响因子：
7.4
通讯作者：
Ruiz Encinar, Jorge
中科院分区：
文献类型：
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作者：
Calderon-Celis, Francisco;Sugiyama, Naoki;Ruiz Encinar, Jorge
目前迫切需要能够提供生物分子，特别是蛋白质及其翻译后修饰的绝对定量的工具，而不需要合适的特定标准。为此，我们显著改进了最近引入的基于CH4加入血浆的策略，使用HPLC-ICP-MS对生物分子进行绝对定量。经过优化，最终选择添加二氧化碳作为一种更安全、更有效的定量策略，能够提供恒定的(
Tools that provide absolute quantification of biomolecules, particularly of proteins and their post-translational modifications, without needing suitable specific standards, are urgently demanded nowadays. To this end, we have significantly improved the recently introduced strategy based on CH4 addition to the plasma for absolute quantification of biomolecules using HPLC-ICP-MS. Addition of CO2 has been optimized and finally selected as a safer, more efficient quantitative strategy that is able to provide constant (