Complete nucleotide sequence of Tn10
Complete nucleotide sequence of Tn10
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DOI：
10.1128/jb.182.10.2970-2972.2000
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发表时间：
2000-05-01
影响因子：
3.2
通讯作者：
Crellin, P
中科院分区：
文献类型：
--
作者：
Chalmers, R;Sewitz, S;Crellin, P
Tn10 的完整核苷酸序列已确定。序列的二核苷酸特征和 G+C 百分比没有不连续性，表明 Tn10 构成同质单位。新序列包含三个新的开放阅读框，对应于谷氨酸通透酶、重金属抗性操纵子的阻遏物以及枯草芽孢杆菌中的假设蛋白质。谷氨酸通透酶在表达时具有完整的功能，但 Tn10 不能保护大肠杆菌免受各种金属的毒性作用。
The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the toxic effects of various metals.