QTL mapping for salt tolerance based on snp markers at the seedling stage in maize (Zea mays L.)
QTL mapping for salt tolerance based on snp markers at the seedling stage in maize (Zea mays L.)
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基于玉米苗期snp标记的耐盐性QTL定位（Zea mays L.）
DOI：
10.1007/s10681-014-1250-x
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发表时间：
2015-05-01
期刊：
影响因子：
1.9
通讯作者：
Zhao, Li
中科院分区：
文献类型：
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作者：
Cui, Dezhou;Wu, Dandan;Zhao, Li
盐碱是玉米种植可持续性和扩大的主要制约因素。植物耐盐性是由多基因控制的数量性状。本研究以161个耐盐性不同的玉米自交系杂交的F2：5重组自交系群体为材料，利用高质量的SNP标记构建了高密度遗传图谱。在盐碱地和水培条件下进行了QTL分析。对于盐碱地，以田间发芽率和田间耐盐性排序为耐盐性指标进行QTL检测。对于水培，耐盐性排名，地上部鲜重，地上部干重，组织含水量，地上部Na+浓度，地上部K+浓度，和地上部K+/Na+比。通过非条件QTL分析，共检测到20个加性QTL和9个上位性QTL，其中12个QTL和2个QTL的处理（Q × T）互作效应显著。此外，利用条件分析模型检测到9个条件QTL。QTL主要集中在第1、3和5染色体上。其中5个非条件QTL和3个条件QTL可以单独解释20%以上的表型变异。这些QTL的发现有助于利用分子标记辅助选择技术改良玉米耐盐性，并为玉米耐盐性的遗传基础研究提供新的思路。
Salinity is a major constraint to the sustainability and expansion of maize cultivation. Plant salt tolerance is a quantitative trait controlled by multiple genes. In the present study, we constructed a high density genetic map based on high quality SNP markers from 161 F2:5 recombinant inbred line populations derived from the cross between two maize inbred lines contrasting in salinity tolerance. QTL analysis was conducted in saline field and the hydroponic culture. For saline field, field germination rate and field salt tolerance ranking were used as salinity tolerance indicators to conduct QTL detection. For hydroponic culture, salt tolerance ranking, shoot fresh weight, shoot dry weight, tissue water content, shoot Na+ concentration, shoot K+ concentration, and shoot K+/Na+ ratio were used. Through unconditional QTL analysis, we detected 20 additive and nine epistatic QTLs, of which 12 and two showed significant QTL by treatment (Q × T) interaction effects, respectively. Moreover, the use of conditional analysis model allowed us to detect nine conditional QTLs. The QTLs were mainly clustered on chromosomes 1, 3 and 5. The five unconditional and three conditional QTLs reported here could individually explain more than 20 % of the phenotypic variation. The QTLs identified here could be helpful to improve salt tolerance in maize by marker-assisted selection and shed new light on understanding the genetic basis of salt tolerance in maize.