Fluorescence lifetime detection in turbid media using spatial frequency domain filtering of time domain measurements.

Fluorescence lifetime detection in turbid media using spatial frequency domain filtering of time domain measurements.
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DOI:
10.1364/ol.38.001440
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发表时间:
2013-05-01
期刊:
影响因子:
3.6
通讯作者:
Kumar AT
Kumar AT
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Kumar AT

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结果表明,时域荧光信号的高空间频率滤波可以有效地检测浑浊介质中的本征荧光寿命,并抑制漫射激发泄漏。该方法的基础是将漫射荧光信号分离为具有不同时空行为的漫射和荧光组分。
It is demonstrated that high spatial frequency filtering of time domain fluorescence signals can allow efficient detection of intrinsic fluorescence lifetimes from turbid media and the rejection of diffuse excitation leakage. The basis of this approach is the separation of diffuse fluorescence signals into diffuse and fluorescent components with distinct spatiotemporal behavior.