THE USE OF PALMITATE-CONJUGATED PROTEIN-A FOR COATING CELLS WITH ARTIFICIAL RECEPTORS WHICH FACILITATE INTERCELLULAR INTERACTIONS
THE USE OF PALMITATE-CONJUGATED PROTEIN-A FOR COATING CELLS WITH ARTIFICIAL RECEPTORS WHICH FACILITATE INTERCELLULAR INTERACTIONS
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DOI：
10.1016/0022-1759(93)90258-9
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发表时间：
1993-01-14
影响因子：
2.2
通讯作者：
PEACOCK, JS
中科院分区：
文献类型：
--
作者：
KIM, SA;PEACOCK, JS
描述了一种方法，其中将棕榈酸酯缀合的蛋白A（pal-蛋白A）掺入到细胞膜上以将抗体锚在细胞表面上。通过将疏水棕榈酸酯部分插入质膜的磷脂双层中，使PAL-蛋白A稳定在细胞表面上。这种膜相关的pal蛋白A保留其对兔免疫球蛋白G（IgG）分子的Fc区的结合亲和力。使用该系统，可以用具有所需特异性的抗体（Ab）分子包被细胞，所述抗体（Ab）分子可以充当可溶性或细胞表面抗原（Ag）的人工受体。在这份报告中，我们表明，A22. E10 T杂交瘤细胞与兔抗小鼠IgG（RaMIgG）包被表现出增加的粘附表面IgG阳性的小鼠B细胞。与包被有非特异性人工受体的细胞相比，在包被有特异性人工受体的细胞中观察到高水平的异型细胞间结合。
A method is described in which palmitate-conjugated protein A (pal-protein A) incorporated onto cell membranes is used to anchor antibodies on the surface of cells. Pal-protein A was stabilized on the cell surfaces via insertion of the hydrophobic palmitate moieties into the phospholipid bilayer of the plasma membrane. This membrane-associated pal-protein A retained its binding affinity for the Fc region of rabbit immunoglobulin G (IgG) molecules. Using this system, cells can be coated with antibody (Ab) molecules of a desired specificity that can serve as artificial receptors for soluble or cell surface antigens (Ags). In this report, we show that A22.E10 T hybridoma cells coated with rabbit anti-mouse IgG (RaMIgG) exhibited increased adhesiveness to surface IgG positive mouse B cells. A high level of heterotypic intercellular conjugation was observed in cells coated with specific artificial receptors as compared to cells coated with nonspecific artificial receptors.