Elevated temperature effects on the oxidant/antioxidant balance in submerged batch cultures of the filamentous fungus Aspergillus niger B1-D
Elevated temperature effects on the oxidant/antioxidant balance in submerged batch cultures of the filamentous fungus Aspergillus niger B1-D
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DOI：
10.1002/bit.10726
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发表时间：
2003-09-30
影响因子：
3.8
通讯作者：
McNeil, B
中科院分区：
文献类型：
--
作者：
Bai, ZH;Harvey, LM;McNeil, B
在本研究中，在尼日尔曲霉B1-D中检查了氧化应激与升高的培养温度之间的关系。 For the first time, both the intracellular levels of the main stressor species (superoxide radical O-2 (-)]) and activities of cellular defensive enzymes (superoxide dismutase [SOD], catalase [CAT], and glutathione peroxide [GPxD were quantified at varying temperature (25, 30, 35, 40degreesC) to more fully characterize culture response in different growth phases. Elevated culture temperature led to increased O-2（.-）在各个培养阶段中的水平。 （50％富集）与在空气中加热的35级培养相比，O-2（ - ）的水平降低，这可能是由于替代真菌呼吸道途径的活性增强所致。我们的发现表明，虽然升高的培养温度确实清楚地诱导了氧化应激事件，但实际上，它通过比以前的研究表明的更为复杂的途径进行了氧化应激事件。温度升高导致SOD和CAT活性的显着差异，这与氧化应激的响应这些酶的活性综合增加非常不同。 （c）2003 Wiley期刊。
In the present study the relationship between oxidative stress and elevated culture temperature was examined in an industrially relevant fungal culture, Aspergillus niger B1-D. For the first time, both the intracellular levels of the main stressor species (superoxide radical O-2 (-)]) and activities of cellular defensive enzymes (superoxide dismutase [SOD], catalase [CAT], and glutathione peroxide [GPxD were quantified at varying temperature (25, 30, 35, 40degreesC) to more fully characterize culture response in different growth phases. Elevated culture temperature led to increased O-2(.-) levels in various culture phases. In the exponential phase this was due to an enhanced generation of O-2(-) whereas in stationary phase a decreased dismutation rate may also have contributed. CAT activities generally increased with culture temperature, whereas GPx activity changed little as temperature rose, indicating that GPx played only a minor role in destroying H2O2 in this A. niger. The combination of elevated temperature (35degreesC) and increased O-2 Supply (50% enrichment) led to decreased levels of O-2(-) compared to the cultivation at 35degreesC gassed with air, probably due to enhanced activity of the alternative fungal respiratory pathway. Our findings indicate that while elevated cultivation temperature does clearly induce oxidative stress events, mechanistically, it does so by a rather more complex route than previous studies indicate. Elevated temperature caused a marked disparity in the activities of SOD and CAT, very distinct from the integrated increase in activity of these enzymes in response to oxidative stress. (C) 2003 Wiley Periodicals.