A rapid Orthopoxvirus purification protocol suitable for high-containment laboratories.
A rapid Orthopoxvirus purification protocol suitable for high-containment laboratories.
复制标题
DOI：
10.1016/j.jviromet.2017.01.018
复制
发表时间：
2017-05
影响因子：
3.1
通讯作者：
Satheshkumar, P. S.
中科院分区：
文献类型：
--
作者：
Hughes, Laura;Wilkins, Kimberly;Goldsmith, Cynthia S.;Smith, Scott;Hudson, Paul;Patel, Nishi;Karem, Kevin;Damon, Inger;Li, Yu;Olson, Victoria A.;Satheshkumar, P. S.
Virus purification in a high-containment setting provides unique challenges due to barrier precautions and operational safety approaches that are not necessary in lower biosafety level (BSL) 2 environments. The need for high risk group pathogen diagnostic assay development, anti-viral research, pathogenesis and vaccine efficacy research necessitates work in BSL-3 and BSL-4 labs with infectious agents. When this work is performed in accordance with BSL-4 practices, modifications are often required in standard protocols. Classical virus purification techniques are difficult to execute in a BSL-3 or BSL-4 laboratory because of the work practices used in these environments. Orthopoxviruses are a family of viruses that, in some cases, requires work in a high-containment laboratory and due to size do not lend themselves to simpler purification methods. Current CDC purification techniques of orthopoxviruses uses 1,1,2-trichlorotrifluoroethane, commonly known as Genetron®. Genetron® is a chlorofluorocarbon (CFC) that has been shown to be detrimental to the ozone and has been phased out and the limited amount of product makes it no longer a feasible option for poxvirus purification purposes. Here we demonstrate a new Orthopoxvirus purification method that is suitable for high-containment laboratories and produces virus that is not only comparable to previous purification methods, but improves on purity and yield.
登录
查看更多内容
DOI：
10.1007/978-1-61779-876-4_4
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Kremer, Melanie;Volz, Asisa;Sutter, Gerd
通讯作者：
Sutter, Gerd
影响因子：
4.4
作者：
Kleiner M;Hooper LV;Duerkop BA
通讯作者：
Duerkop BA
影响因子：
12.7
作者：
Berting, A;Goerner, W;Kreil, TR
通讯作者：
Kreil, TR
影响因子：
5.4
作者：
VER, BA;MELNICK, JL;WALLIS, C
通讯作者：
WALLIS, C
影响因子：
3.8
作者：
HEINRICY, U;STIERHOF, YD;FLEHMIG, B
通讯作者：
FLEHMIG, B