Rerouting of Plant Late Endocytic Trafficking Toward a Pathogen Interface
Rerouting of Plant Late Endocytic Trafficking Toward a Pathogen Interface
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DOI：
10.1111/tra.12245
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发表时间：
2015-02-01
期刊：
影响因子：
4.5
通讯作者：
Kamoun, Sophien
中科院分区：
文献类型：
--
作者：
Bozkurt, Tolga O.;Belhaj, Khaoula;Kamoun, Sophien
许多植物病原微生物和共生微生物产生特殊的细胞结构，侵入宿主细胞，并被宿主源膜包裹。宿主-微生物界面的生物发生和功能机制尚不清楚。在这里，我们发现植物晚期的内吞运输被转移到吸器外膜（EHM）；在爱尔兰马铃薯饥荒病原体疫霉入侵的植物细胞中发育的一种宿主-病原体界面。一个晚期核内体和tono质体标记蛋白Rab7 GTPase RabG3c，而不是tono质体定位的蔗糖转运蛋白，被招募到EHM中，这表明液泡靶向的晚期核内体特异性重定向到宿主-病原体界面。我们通过显示激活后，细胞表面免疫受体向吸器界面运输，揭示了这一过程的动态性质。我们的工作为EHM的生物发生提供了见解，并揭示了将膜室和免疫受体招募到这个宿主-病原体界面的动态过程。
A number of plant pathogenic and symbiotic microbes produce specialized cellular structures that invade host cells where they remain enveloped by host-derived membranes. The mechanisms underlying the biogenesis and functions of host-microbe interfaces are poorly understood. Here, we show that plant late endocytic trafficking is diverted toward the extrahaustorial membrane (EHM); a host-pathogen interface that develops in plant cells invaded by Irish potato famine pathogen Phytophthora infestans. A late endosome and tonoplast marker protein Rab7 GTPase RabG3c, but not a tonoplast-localized sucrose transporter, is recruited to the EHM, suggesting specific rerouting of vacuole-targeted late endosomes to a host-pathogen interface. We revealed the dynamic nature of this process by showing that, upon activation, a cell surface immune receptor traffics toward the haustorial interface. Our work provides insight into the biogenesis of the EHM and reveals dynamic processes that recruit membrane compartments and immune receptors to this host-pathogen interface.