Sex differences and hormonal effects on gut microbiota composition in mice.
Sex differences and hormonal effects on gut microbiota composition in mice.
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DOI：
10.1080/19490976.2016.1203502
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发表时间：
2016-07-03
期刊：
影响因子：
12.2
通讯作者：
Lusis AJ
中科院分区：
文献类型：
--
作者：
Org E;Mehrabian M;Parks BW;Shipkova P;Liu X;Drake TA;Lusis AJ
我们之前报道了一组89种不同近交系小鼠肠道微生物群的定量，现在我们研究了微生物群组成的性别差异问题。当一起检查689只小鼠的总种群时，几个分类群在性别之间的丰度上表现出显着差异，但在单个品系水平上观察到了更大数量的差异，这表明性别差异可能会被宿主遗传和环境因素掩盖。我们还检查了一组喂食食物和高脂肪饮食的小鼠，并观察了性别与饮食的相互作用。我们进一步研究了性别差异使用性腺切除和激素治疗的小鼠从3个不同的近交系。未加权UniFrac距离的主坐标分析显示，在所有3种菌株中，性腺切除术和激素替代对微生物群组成的影响非常明显。此外，胆汁酸分析显示了性别特异性差异以及性腺切除术的影响，提供了一种可能的机制介导微生物群组成的性别差异。
We previously reported quantitation of gut microbiota in a panel of 89 different inbred strains of mice, and we now examine the question of sex differences in microbiota composition. When the total population of 689 mice was examined together, several taxa exhibited significant differences in abundance between sexes but a larger number of differences were observed at the single strain level, suggesting that sex differences can be obscured by host genetics and environmental factors. We also examined a subset of mice on chow and high fat diets and observed sex-by-diet interactions. We further investigated the sex differences using gonadectomized and hormone treated mice from 3 different inbred strains. Principal coordinate analysis with unweighted UniFrac distances revealed very clear effects of gonadectomy and hormone replacement on microbiota composition in all 3 strains. Moreover, bile acid analyses showed gender-specific differences as well as effects of gonodectomy, providing one possible mechanism mediating sex differences in microbiota composition.