Identification of human glandular kallikrein in the beta cell of the pancreas.
Identification of human glandular kallikrein in the beta cell of the pancreas.
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胰腺β细胞中人腺激肽释放酶的鉴定。
DOI：
10.1056/nejm197906073002301
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
K. Austen
中科院分区：
文献类型：
--
作者：
O. ole;G. Pinkus;J. Spragg;K. Austen
为了确定腺激肽释放酶在人胰腺中的细胞定位，使用针对抗原相同的尿激肽释放酶（尿激肽释放酶）的单特异性抗体进行免疫组织化学研究。腺胰腺激肽释放酶的胰岛β细胞的本地化是相同的胰岛素在正常人胰腺和两个胰岛细胞肿瘤。当胰岛细胞肿瘤或青少年糖尿病患者的胰腺中缺乏β细胞时，则检测不到激肽释放酶抗原。用纯化的尿或胰腺激肽释放酶吸收的抗尿激肽释放酶不再识别胰腺抗原，而用胰岛素吸收则没有影响。人胰激肽释放酶是一种内肽酶，与羧肽酶B一起将牛胰岛素原转化为具有胰岛素电泳迁移率的多肽，其β细胞定位表明胰激肽释放酶可能参与胰岛素原的生理激活。
To determine the cellular localization of glandular kallikrein in the human pancreas, immunohistochemical studies were performed with a monospecific antibody against the antigenically identical urinary kallikrein (urokallikrein). The localization of glandular pancreatic kallikrein to the beta cells of the islets was the same as that of insulin in normal human pancreas and in two islet-cell tumors. When beta cells were lacking in islet-cell tumors or in the pancreas of a patient with juvenile-onset diabetes, kallikrein antigen was not detectable. Anti-urokallikrein absorbed with purified urinary or pancreatic kallikrein no longer identified a pancreatic antigen, whereas absorption with insulin had no effect. The beta-cell localization of human pancreatic kallikrein, an endopeptidase that, in concert with carboxypeptidase B, converts bovine proinsulin to a polypeptide with the electrophoretic mobility of insulin, suggests that pancreatic kallikrein may be involved in the physiologic activation of proinsulin.