Amoxicillin-resistant oral streptococci identified in dental plaque specimens from healthy Japanese adults.
Amoxicillin-resistant oral streptococci identified in dental plaque specimens from healthy Japanese adults.
复制标题
在健康日本成年人的牙菌斑标本中发现了对阿莫西林耐药的口腔链球菌。
DOI：
10.1016/j.jjcc.2011.12.004
复制
发表时间：
2012
影响因子：
2.5
通讯作者：
Ooshima T
中科院分区：
文献类型：
--
作者：
Masuda K;Nemoto H;Nakano K;Naka S;Nomura R;Ooshima T
背景：有效性心内膜炎（IE）被认为是一种危及生命的疾病，侵入性牙科手术被认为是重要因素。口服阿莫西林（AMPC）广泛用于有IE风险的心脏病患者的预防。然而，关于AMPC对口腔细菌的抑制作用的信息有限。方法与结果从120名健康的日本成年受试者中获得牙菌斑标本，稀释后在口腔链球菌选择性培养基上染色。AMPC的最低抑菌浓度（MIC）由临床实验室标准研究所（2006）采用宏稀释法测定。从5名受试者中分离到7株AMPC MIC≥16μg/mL的菌株。各菌株的16S rRNA序列分析证实菌株属血链球菌最多，其次为口腔链球菌。每隔2 ~ 3个月再次采集5例患者的牙菌斑标本，均未发现AMPC MIC≥16μg/mL的菌株。结论高MIC的AMPC菌株存在于日本成人口腔中，但它们可能是短暂的，而不是常驻的。
BACKGROUNDInfective endocarditis (IE) is known to be a life-threatening disease and invasive dental procedures are considered to be important factors. Oral amoxicillin (AMPC) is widely used for prophylaxis in patients with heart disorders who are at risk for IE. However, there is only limited information regarding the inhibition of oral bacteria by AMPC.METHODS AND RESULTSDental plaque specimens were obtained from 120 healthy Japanese adult subjects, then diluted and streaked onto selective medium for oral streptococci. The minimum inhibitory concentration (MIC) of AMPC was evaluated using a macro-dilution method by Clinical Laboratory Standard Institute (2006). Seven strains with an MIC of AMPC of 16μg/mL or more were isolated from 5 subjects. The bacterial species were confirmed by sequence analysis of 16S rRNA from each strain, which demonstrated that most were Streptococcus sanguinis, followed by Streptococcus oralis. Dental plaque specimens collected from these 5 subjects again after an interval of 2–3 months possessed no strains with an MIC of AMPC of 16μg/mL or more.CONCLUSIONSThese findings suggest that strains with a high MIC of AMPC are present in the oral cavities of Japanese adults, though they may be transient rather than inhabitants.