POSSIBLE TEMPERATURE-DEPENDENT BLOCKAGE OF SYNAPTIC VESICLE RECYCLING INDUCED BY A SINGLE GENE MUTATION IN DROSOPHILA
POSSIBLE TEMPERATURE-DEPENDENT BLOCKAGE OF SYNAPTIC VESICLE RECYCLING INDUCED BY A SINGLE GENE MUTATION IN DROSOPHILA
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DOI：
10.1002/neu.480140305
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
IKEDA, K
中科院分区：
文献类型：
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作者：
KOSAKA, T;IKEDA, K
对温度敏感的果蝇突变体shibiretsl（shi）在30 ℃时表现出突触传递的可逆阻滞。C.电镜观察到该突变体的神经肌肉、感觉、中枢等突触终末。在19度。C时，shi的所有末端显示出与野生型（Oregon-R）果蝇基本相同的结构特征，而在30 ℃时，shi的所有末端显示出与野生型果蝇基本相同的结构特征。C（5或10分钟的曝光）shi终端表现出各种结构的变化，没有看到野生型。在所有不同类型的终端中观察到的主要结构变化是突触前位点附近的质膜上的许多坑状结构的积累。这些结构由直径约50-100 nm的球形头部和约20 nm长、直径约20-25 nm的圆柱形颈部组成。颈部被一种细胞质致密物质包围，约10 nm厚，使人联想到一个衣领。这些坑被称为领坑。在19 ℃的野生型果蝇中观察到类似类型的凹坑，尽管非常罕见。30度。在19.degree.的温度下飞行。C.不同程度的小泡消耗，和膜结构（内折和池状或管状结构）的增加往往伴随着纹孔的形成。这些凹坑可能是内吞过程中受阻步骤的结果，这反过来又导致囊泡耗尽，因为胞吐作用进行。
The temperature-sensitive Drosophila mutant, shibiretsl (shi), exhibits a reversible block in synaptic transmission at 30.degree. C. Various synaptic terminals (neuromuscular, sensory, central) of this mutant were observed by EM. At 19.degree. C, all terminals of shi showed essentially the same structural features as those of wild-type (Oregon-R) flies, while at 30.degree. C (5 or 10 min of exposure) shi terminals exhibited various structural changes not seen in the wild type. The major structural change observed in all of the various types of terminals was the accumulation of many pitlike structures on the plasma membrane near presynaptic sites. These structures consisted of a spherical head portion, about 50-100 nm in diameter, and a cylindrical neck portion, about 20 nm long and 20-25 nm in diameter. The neck portion was surrounded by a kind of cytoplasmic dense material, about 10 nm thick, reminiscent of a collar. These pits are referred to as collared pits. Similar kinds of pits were observed, although very rarely, in wild-type flies at 19.degree. and 30.degree. C and in shi flies at 19.degree. C. Various degrees of vesicle depletion, and an increase in membranous structures (infoldings and cisternalike or tubulelike structures) often accompanied pit formation. These pits may be the result of a blocked step in the endocytotic process, which in turn causes vesicle depletion as exocytosis proceeds.