Engineering aptazyme switches for conditional gene expression in mammalian cells utilizing an in vivo screening approach.
Engineering aptazyme switches for conditional gene expression in mammalian cells utilizing an in vivo screening approach.
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利用体内筛选方法工程改造适体酶开关以在哺乳动物细胞中进行条件基因表达
DOI：
10.1007/978-1-4939-2730-2_11
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发表时间：
2015
影响因子：
--
通讯作者：
Hartig JS
中科院分区：
文献类型：
--
作者：
Rehm C;Klauser B;Hartig JS
人工RNA开关是一类适用于合成生物学应用的新兴遗传控制器。适体酶是由适体结构域和自裂核酶组成的融合体。与传统的转录因子技术相比，利用适体酶进行条件基因表达显示出几个优势，因为适体酶需要最小的基因组空间，不需要蛋白质辅助因子就能实现其功能，最重要的是，适体酶在配体选择性和RNA功能方面是可重编程的。能够根据输入配体生成适配酶的技术对生物计算设备和生物传感应用的构建很感兴趣。在本章中，我们提出了一种方法，便于在哺乳动物细胞中随机筛选适配酶池。
Artificial RNA switches are an emerging class of genetic controllers suitable for synthetic biology applications. Aptazymes are fusions composed of an aptamer domain and a self-cleaving ribozyme. The utilization of aptazymes for conditional gene expression displays several advantages over employing conventional transcription factor-based techniques as aptazymes require minimal genomic space, fulfill their function without the need of protein cofactors, and most importantly are reprogrammable with respect to ligand selectivity and the RNA function to be regulated. Technologies that enable the generation of aptazymes to defined input ligands are of interest for the construction of biocomputing devices and biosensing applications. In this chapter we present a method that facilitates the in vivo screening of randomized pools of aptazymes in mammalian cells.
影响因子：
16.6
作者：
Wieland, Markus;Hartig, Joerg S.
通讯作者：
Hartig, Joerg S.
人工核糖开关：基于合成 mRNA 的基因表达调节剂
影响因子：
3.2
作者：
Markus Wieland;J. Hartig
通讯作者：
J. Hartig