Genetic diversity of the natural populations of Arabidopsis thaliana in China
Genetic diversity of the natural populations of Arabidopsis thaliana in China
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DOI：
10.1038/sj.hdy.6801020
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发表时间：
2007-10-01
期刊：
影响因子：
3.8
通讯作者：
H Gu
中科院分区：
文献类型：
--
作者：
F He;D Kang;H Gu
虽然国内外已对拟南芥种群的遗传结构进行了广泛的研究，但中国种群的遗传结构尚未被研究过。在本研究中，我们采集了分布于长江下游中国东部的19个太子参自然居群和新疆西北部中国的2个居群，共计560只。采用简单序列间重复序列(ISSRs)和随机扩增多态性DNA(RAPDS)两种分子标记，研究了不同居群内和居群间的遗传多样性以及遗传距离和地理距离之间的相关性。13个ISSR引物共扩增出165条多态条带(PPB)，占96%；11个随机引物扩增出162条(98%)多态带，共扩增出约560个个体。这两个标记系统在这些自然种群中产生了相似的遗传多样性模式。AMOVA分析表明，大约42%-45%的遗传变异存在于种群内，并发现了可能的地理结构。Mantel测试显示，地理距离和这些种群的总体遗传距离之间存在显著的相关性。江西4个居群间亲缘关系较近，在ISSR和RAPD数据的算术平均聚类图上，江西4个居群往往聚为一个单系群。根据自然分布种群的再定殖化和灭绝及其遗传分化格局的观察，该种在中国的分布可能是在强烈的人类活动影响下的自然扩散的结果。
Although extensive studies have been conducted on the genetic structure of Arabidopsis thaliana ( A. thaliana) populations worldwide, the populations from China have never been studied. In this study, we collected 560 individuals from 19 natural populations of A. thaliana distributed in East China along the lower reaches of the Yangtze River, and two populations from northwest China ( Xinjiang Province). We adopted two kinds of molecular marker, inter-simple sequence repeats (ISSRs) and random amplified polymorphic DNA (RAPDs) to investigate the genetic diversity within and among populations, and the correlation between the genetic and geographic distances. Thirteen ISSR primers produced 165 polymorphic bands (PPB) (96%) and 11 RAPD primers produced 162 polymorphic bands (98%) in about 560 individuals. The two marker systems generated similar patterns of genetic diversity in these natural populations. The AMOVA analysis indicated about 42-45% of the total genetic variation existed within populations, and found possible geographic structure. The Mantel test revealed a significant correlation between the geographic distance and the genetic distance of these populations in general. A close genetic relationship was found among four populations in the Jiangxi Province, and these always appeared clustered together as a monophyletic group in unweighted pair-group method with arithmetic averages dendrograms based on both ISSR and RAPD data sets. Based on the observation of recolonization and extinction of naturally distributed populations of A. thaliana, and the pattern of their genetic differentiation, the distribution of this species in China might be a result of natural dispersal under the strong influence of human activity.