Hepatoprotective effect of parthenolide in rat model of nonalcoholic fatty liver disease
Hepatoprotective effect of parthenolide in rat model of nonalcoholic fatty liver disease
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DOI：
10.1080/08923973.2017.1327965
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发表时间：
2017-01-01
影响因子：
3.3
通讯作者：
Khodadadi, Iraj
中科院分区：
文献类型：
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作者：
Bahabadi, Majid;Mohammadalipour, Adel;Khodadadi, Iraj
背景：传统中药的有效成分一直是科学界关注的焦点。目的：探讨parthenolide治疗非酒精性脂肪性肝病（NAFLD）的作用机制。材料与方法：35只雄性Wistar大鼠分别饲喂高脂饲料（HFD） 8周，腹腔注射或不注射孤雌醇内酯（parthenolide）观察NAFLD的发生。测定肝脏甘油三酯（TG）、总抗氧化能力（TAC）、总氧化状态（TOS）、硫代巴比妥酸反应物（TBARs）、总巯基、肿瘤坏死因子α (tnf - α)和细胞色素P4502E1 （CYP2E1）水平以及肝脏ALT、AST和过氧化氢酶活性。此外，采用实时荧光定量PCR检测肝脏中tnf - α、CYP2E1和核因子κ B （nf - κ B）基因表达水平。结果：HFD导致体重显著增加，肝脏TG含量增加，ALT和AST活性改变，在给药后减弱(p
Context: The active ingredients of traditional medical herbs have been the focus of scientific interests.Objective: This study was designed to explore the mechanisms of actions of parthenolide on nonalcoholic fatty liver disease (NAFLD).Materials and methods: Thirty-five male Wistar rats were fed high-fat diet (HFD) for eight weeks with or without an intraperitoneal injection of parthenolide to develop NAFLD. Liver triacylglycerol (TG), total antioxidant capacity (TAC), total oxidative status (TOS), thiobarbituric acid reactant substances (TBARs), total thiol groups and tumor necrosis factor alpha (TNF-alpha) and cytochrome P4502E1 (CYP2E1) levels as well as liver ALT, AST and catalase activities were determined. In addition, quantitative real-time PCR was performed to obtain hepatic gene expression levels of TNF-alpha, CYP2E1 and nuclear factor-kappa B (NF-kappa B).Results: HFD caused a significant weight gain and increased liver TG content as well as alteration in ALT and AST activities, which were attenuated after administration of parthenoide (p