The hibernating South American marsupial, Dromiciops gliroides, displays torpor-sensitive microRNA expression patterns.
The hibernating South American marsupial, Dromiciops gliroides, displays torpor-sensitive microRNA expression patterns.
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DOI：
10.1038/srep24627
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发表时间：
2016-04-19
影响因子：
4.6
通讯作者：
Storey KB
中科院分区：
文献类型：
--
作者：
Hadj-Moussa H;Moggridge JA;Luu BE;Quintero-Galvis JF;Gaitán-Espitia JD;Nespolo RF;Storey KB
当面对不利的环境条件时，有袋类动物Dromiciops gliroides使用日常或季节性的麻痹来支持生存，是南美洲唯一已知的冬眠哺乳动物。作为古代微生兽目的唯一活着的代表，这个物种可以提供关于冬眠的进化起源和生化机制的重要信息。冬眠是一种复杂的节能策略，涉及部分由microRNA引起的基因表达变化。为了更好地阐明microRNA在协调低代谢中的作用，采用改良的茎环技术和定量PCR来表征对照和迟钝D的肝脏和骨骼肌中85个microRNA的相对表达水平。神经胶质瘤39种microRNA在麻痹期间差异调节;其中，35种在肝脏中下调，11种在骨骼肌中差异表达。生物信息学分析预测，下调的肝脏microRNA与MAPK、PI 3 K-Akt和mTOR通路的激活相关，表明它们在促进有袋动物麻痹中的重要性。在骨骼肌中，冬眠反应microRNA被预测为调节粘着斑、ErbB和mTOR通路，表明促进肌肉维持机制。这些组织特异性反应表明，microRNA调节促进冬眠，体温调节和预防肌肉废用性萎缩的关键分子途径。
When faced with adverse environmental conditions, the marsupial Dromiciops gliroides uses either daily or seasonal torpor to support survival and is the only known hibernating mammal in South America. As the sole living representative of the ancient Order Microbiotheria, this species can provide crucial information about the evolutionary origins and biochemical mechanisms of hibernation. Hibernation is a complex energy-saving strategy that involves changes in gene expression that are elicited in part by microRNAs. To better elucidate the role of microRNAs in orchestrating hypometabolism, a modified stem-loop technique and quantitative PCR were used to characterize the relative expression levels of 85 microRNAs in liver and skeletal muscle of control and torpid D. gliroides. Thirty-nine microRNAs were differentially regulated during torpor; of these, 35 were downregulated in liver and 11 were differentially expressed in skeletal muscle. Bioinformatic analysis predicted that the downregulated liver microRNAs were associated with activation of MAPK, PI3K-Akt and mTOR pathways, suggesting their importance in facilitating marsupial torpor. In skeletal muscle, hibernation-responsive microRNAs were predicted to regulate focal adhesion, ErbB, and mTOR pathways, indicating a promotion of muscle maintenance mechanisms. These tissue-specific responses suggest that microRNAs regulate key molecular pathways that facilitate hibernation, thermoregulation, and prevention of muscle disuse atrophy.