3-DIMENSIONAL MORPHOMETRICAL STUDY OF DENDRITIC SPINES OF THE GRANULE CELL IN THE RAT DENTATE GYRUS WITH HVEM STEREO IMAGES
3-DIMENSIONAL MORPHOMETRICAL STUDY OF DENDRITIC SPINES OF THE GRANULE CELL IN THE RAT DENTATE GYRUS WITH HVEM STEREO IMAGES
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DOI：
10.1002/jemt.1060120203
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发表时间：
1989-06-01
期刊：
影响因子：
--
通讯作者：
KOSAKA, T
中科院分区：
文献类型：
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作者：
HAMA, K;ARII, T;KOSAKA, T
借助三维图像分析系统，通过5 m厚高尔基体标本的高压电子显微镜立体图像，测量大鼠齿状回背叶颗粒细胞树突棘的数量、长度和直径。棘密度分别为2.02 ± 0.28、2.28 ± 0.33和3.36± 0.35/1 μm，并获得枝晶的近端部分。这些值约为之前光学显微镜报告的1.6倍。远端、中部和近端的平均三维脊柱长度分别为1.244 ± 0.506 μm、1.262 ± 0.563 μm和1.254 ± 0.584 μm，其长度约为二维测量的1.4倍。通过使用测量的棘的形态计量学参数，如长度、直径和种群密度，在远端、中间和近端部分，每1 μm树突的总棘表面积分别为2.401 μm2、2.806 μm2和4.180 μ m2。通过在每个枝晶部分增加刺，枝晶的总表面积增加了约一倍。
Number, length, and diameters of dendritic spines of the granule cell in the dorsal leaf of the rat dentate gyrus were measured by using high‐voltage electron microscope stereo images of 5‐m̈m‐thick Golgi preparations with the aid of a three‐dimensional image analyzer system.Spine densities of 2.02 ± 0.28, 2.28 ± 0.33, and 3.36± 0.35 per 1 μm at distal, middle, and proximal portions of the dendrite were obtained. These values were about 1.6‐fold of the previous light microscopical report.Mean three‐dimensional spine length were 1.244 ± 0.506 μm, 1.262 ± 0.563 μm, and 1.254 ± 0.584 μm at distal, middle, and proximal portions, respectively, which were about 1.4 times longer than those measured in two dimensions.By using measured morphometrical parameters of spines such as lengths, diameters, and population densities, total spine surface areas of 2.401 μm2, 2.806 μm2, and 4.180 μm2per 1 μm of the dendrite at distal, middle, and proximal portions, respectively, were obtained. The total surface area of dendrite was about doubled by the addition of the spines at each dendritic portion.The advantageous features and the problems of the present method are discussed.